ArticleNucleic acids research2005
Conditional knockdown of Fgfr2 in mice using Cre-LoxP induced RNA interference.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 91 citations in OpenAlex.
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- LADD syndrome with glaucoma is caused by a novel gene.Molecular vision · 2017Article
- FGF signaling in the osteoprogenitor lineage non-autonomously regulates postnatal chondrocyte proliferation and skeletal growth.Development (Cambridge, England) · 2016Article
- Fibroblast growth factor signaling in skeletal development and disease.Genes & development · 2015Review
- shRNA off-target effects in vivo: impaired endogenous siRNA expression and spermatogenic defects.PloS one · 2015Article
- Genetically engineered mouse models for drug development and preclinical trials.Biomolecules & therapeutics · 2014Review
- Role of FGF/FGFR signaling in skeletal development and homeostasis: learning from mouse models.Bone research · 2014Review
- Heritable and inducible gene knockdown in astrocytes or neurons in vivo by a combined lentiviral and RNAi approach.Frontiers in cellular neuroscience · 2014Article
- Accelerating cancer modeling with RNAi and nongermline genetically engineered mouse models.Cold Spring Harbor protocols · 2013Review
- Loss of androgen receptor promotes adipogenesis but suppresses osteogenesis in bone marrow stromal cells.Stem cell research · 2013Article
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- Extracellular matrix degradation and remodeling in development and disease.Cold Spring Harbor perspectives in biology · 2011Review
- Construction of permanently inducible miRNA-based expression vectors using site-specific recombinases.BMC biotechnology · 2011Article
- Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin selection cassettes.Biological procedures online · 2011Article
- Essential roles of BCCIP in mouse embryonic development and structural stability of chromosomes.PLoS genetics · 2011Article
- A non-specific effect associated with conditional transgene expression based on Cre-loxP strategy in mice.PloS one · 2011Article
- Disrupted junctional membrane complexes and hyperactive ryanodine receptors after acute junctophilin knockdown in mice.Circulation · 2011Article
- GEP, a local growth factor, is critical for odontogenesis and amelogenesis.International journal of biological sciences · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
RNA interference (RNAi)-mediated gene knockdown is a potent approach for studying gene function. We have previously reported a plasmid-based, tamoxifen-inducible gene knockdown system in cultured cells using a combined RNAi and Cre-LoxP system. Here, we validate this system in mouse and show that it can be used to suppress the expression of an endogenous gene (Fgfr2) with high efficiency. We show that transgenic mice carrying the U6-ploxPneo-Fgfr2 RNAi construct are normal, displaying Fgfr2 transcripts equivalent to those of wild-type controls, indicating that the U6 promoter is inactive in vivo due to the presence of the neo in the promoter. After excision of the neo by crossing with transgenic mice that express Cre in the mouse germline, the U6 promoter is activated, leading to over 95% reduction of Fgfr2 transcripts, and consequently, embryonic lethality. On the other hand, activation of the U6 promoter using transgenic mice that express Cre in the progress zone of the limb results in live mice with malformation of digits of both the forelimbs and hindlimbs. This method provides a fast, yet efficient way to decipher gene functions in vivo in a tissue-specific manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.