ArticleProceedings of the National Academy of Sciences of the United States of America2005
Evolution of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase-encoding genes in the yeast Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Endogenous pathway dysregulation in Kluyveromyces marxianus under nitrogen limitation drives 2-phenylethyl acetate and 2-phenylethanol overproduction.Scientific reports · 2025Article
- Screening of High-Yield 2-Phenylethanol Producing Strain from Wild-TypeFoods (Basel, Switzerland) · 2025Article
- Semi-rational design and modification of phosphoketolase to improve the yield of tyrosol inSynthetic and systems biotechnology · 2025Article
- A comprehensive review and comparison of L-tryptophan biosynthesis inFrontiers in bioengineering and biotechnology · 2023Review
- Adaptive evolution in the Saccharomyces kudriavzevii Aro4p promoted a reduced production of higher alcohols.Microbial biotechnology · 2022Article
- Review
- Combining mechanistic and machine learning models for predictive engineering and optimization of tryptophan metabolism.Nature communications · 2020Article
- Article
- A systematic optimization of styrene biosynthesis inBiotechnology for biofuels · 2018Article
- Metabolic Engineering of the Shikimate Pathway for Production of Aromatics and Derived Compounds-Present and Future Strain Construction Strategies.Frontiers in bioengineering and biotechnology · 2018Article
- Enhancement of rapamycin production by metabolic engineering in Streptomyces hygroscopicus based on genome-scale metabolic model.Journal of industrial microbiology & biotechnology · 2017Article
- Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using L-Tyrosine Over-Producing Saccharomyces cerevisiae.PloS one · 2015Article
- Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement.Microbial cell factories · 2013Article
- The diversity of allosteric controls at the gateway to aromatic amino acid biosynthesis.Protein science : a publication of the Protein Society · 2013Review
- Comparative transcriptomics reveals different strategies of Trichoderma mycoparasitism.BMC genomics · 2013Article
- Biosynthesis of cis,cis-muconic acid and its aromatic precursors, catechol and protocatechuic acid, from renewable feedstocks by Saccharomyces cerevisiae.Applied and environmental microbiology · 2012Article
- Biosynthetic and iron metabolism is regulated by thiol proteome changes dependent on glutaredoxin-2 and mitochondrial peroxiredoxin-1 in Saccharomyces cerevisiae.The Journal of biological chemistry · 2011Article
- Tyrosine latching of a regulatory gate affords allosteric control of aromatic amino acid biosynthesis.The Journal of biological chemistry · 2011Article
- Synergistic allostery, a sophisticated regulatory network for the control of aromatic amino acid biosynthesis in Mycobacterium tuberculosis.The Journal of biological chemistry · 2010Article
- Linking high-resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The shikimate pathway resulting in three aromatic amino acids is initiated in different organisms by two and three 3-deoxy-d-arabino-heptulosonate-7-phosphate synthases, respectively. Aro3p and Aro4p are the yeast enzymes feedback-inhibited by phenylalanine and tyrosine, respectively. A yeast strain deficient in the general control transcriptional regulatory system of amino acid biosynthesis is unable to live in the presence of high amounts of phenylalanine and tyrosine. Here, we show that this yeast strain can be rescued by the expression of aroH from Escherichia coli encoding the tryptophan-regulated AroH as third isoenzyme. Yeast carrying Ec AroH as the only enzyme for the initial step of the shikimate pathway can grow in the absence of tryptophan. Without aromatic amino acids, this yeast strain survives only when the yeast ARO3 promoter instead of the ARO4 promoter drives E. coli aroH. The detailed analysis of Aro3p and Aro4p revealed a triple feedback control by tyrosine/phenylalanine and tryptophan. Dissecting this control allowed engineering of Aro4p S195A as an enzyme, which is inhibited like AroH only by tryptophan. In addition, Aro4p variants were constructed that show an equally strong inhibition by tyrosine and tryptophan (Aro4p P165G Q302R) and in which the regulation by tyrosine and tryptophan was reversed (Aro4p P165G). Our data suggest that yeast possesses only two instead of three isogenes encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthases because both isoenzymes can be fine tuned by tryptophan as additional effector and because transcriptional regulation by the general control system can be induced as backup when aromatic amino acids in the environment are imbalanced.
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