ArticleMolecular biology of the cell2005
Multiple myosin II heavy chain kinases: roles in filament assembly control and proper cytokinesis in Dictyostelium.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 87 citations in OpenAlex.
- Actomyosin dynamics in detached cells: linking clutch model to cell migration and cytokinesis.BMC molecular and cell biology · 2025Article
- Complementary cytoskeletal feedback loops control signal transduction excitability and cell polarity.Nature communications · 2025Article
- Multiple Mechanisms to Regulate Actin Functions: "Fundamental" Versus Lineage-Specific Mechanisms and Hierarchical Relationships.Biomolecules · 2025Review
- Traveling-wave chemotaxis of neutrophil-like HL-60 cells.Molecular biology of the cell · 2025Article
- A continuum model of mechanosensation based on contractility kit assembly.Biophysical journal · 2025Article
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- Dynamin-Like Protein B ofCells · 2019Article
- The Cytoskeleton of the Retinal Pigment Epithelium: from Normal Aging to Age-Related Macular Degeneration.International journal of molecular sciences · 2019Review
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Myosin IIB assembly state determines its mechanosensitive dynamics.The Journal of cell biology · 2019Article
- Turnover and flow of the cell membrane for cell migration.Scientific reports · 2017Article
- Structure of the Dictyostelium Myosin-II Heavy Chain Kinase A (MHCK-A) α-kinase domain apoenzyme reveals a novel autoinhibited conformation.Scientific reports · 2016Article
- Cytokinesis: Robust cell shape regulation.Seminars in cell & developmental biology · 2016Review
- Characterization of the Catalytic and Nucleotide Binding Properties of the α-Kinase Domain of Dictyostelium Myosin-II Heavy Chain Kinase A.The Journal of biological chemistry · 2015Article
- Cell shape regulation through mechanosensory feedback control.Journal of the Royal Society, Interface · 2015Article
- Pharmacological activation of myosin II paralogs to correct cell mechanics defects.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Kinetic Monte Carlo simulations of the assembly of filamentous biomacromolecules by dimer addition mechanism.RSC advances · 2015Article
- Genetic suppression of a phosphomimic myosin II identifies system-level factors that promote myosin II cleavage furrow accumulation.Molecular biology of the cell · 2014Article
- A novel alpha kinase EhAK1 phosphorylates actin and regulates phagocytosis in Entamoeba histolytica.PLoS pathogens · 2014Article
- Life without double-headed non-muscle myosin II motor proteins.Frontiers in chemistry · 2014Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Myosin II filament assembly in Dictyostelium discoideum is regulated via phosphorylation of residues located in the carboxyl-terminal portion of the myosin II heavy chain (MHC) tail. A series of novel protein kinases in this system are capable of phosphorylating these residues in vitro, driving filament disassembly. Previous studies have demonstrated that at least three of these kinases (MHCK A, MHCK B, and MHCK C) display differential localization patterns in living cells. We have created a collection of single, double, and triple gene knockout cell lines for this family of kinases. Analysis of these lines reveals that three MHC kinases appear to represent the majority of cellular activity capable of driving myosin II filament disassembly, and reveals that cytokinesis defects increase with the number of kinases disrupted. Using biochemical fractionation of cytoskeletons and in vivo measurements via fluorescence recovery after photobleaching (FRAP), we find that myosin II overassembly increases incrementally in the mutants, with the MHCK A(-)/B(-)/C(-) triple mutant showing severe myosin II overassembly. These studies suggest that the full complement of MHC kinases that significantly contribute to growth phase and cytokinesis myosin II disassembly in this organism has now been identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.