ArticleMolecular biology of the cell2005
Intermediate filaments interact with dormant ezrin in intestinal epithelial cells.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- KRT81 promotes metastasis of colorectal cancer by acting as a protein scaffold for ezrin.Oncogene · 2026Article
- XB130 Deficiency Causes Congenital Hypothyroidism in Mice due to Disorganized Apical Membrane Structure and Function of Thyrocytes.Thyroid : official journal of the American Thyroid Association · 2021Article
- A tensile trilayered cytoskeletal endotube drives capillary-like lumenogenesis.The Journal of cell biology · 2019Article
- Three-dimensional Organization of Layered Apical Cytoskeletal Networks Associated with Mouse Airway Tissue Development.Scientific reports · 2017Article
- Review
- Physical biology in cancer. 5. The rocky road of metastasis: the role of cytoskeletal mechanics in cell migratory response to 3D matrix topography.American journal of physiology. Cell physiology · 2014Review
- Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.The Journal of biological chemistry · 2012Article
- The novel intestinal filament organizer IFO-1 contributes to epithelial integrity in concert with ERM-1 and DLG-1.Development (Cambridge, England) · 2012Article
- Ezrin expression as a prognostic marker in colorectal adenocarcinoma.Pathology oncology research : POR · 2011Article
- Alterations in the proteome of the NHERF2 knockout mouse jejunal brush border membrane vesicles.Physiological genomics · 2011Article
- Lipid rafts and cytoskeletal proteins in placental microvilli membranes from preeclamptic and IUGR pregnancies.The Journal of membrane biology · 2011Article
- skNAC, a Smyd1-interacting transcription factor, is involved in cardiac development and skeletal muscle growth and regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Rescue of atypical protein kinase C in epithelia by the cytoskeleton and Hsp70 family chaperones.Journal of cell science · 2009Article
- Vimentin regulates scribble activity by protecting it from proteasomal degradation.Molecular biology of the cell · 2009Article
- Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium.Molecular biology of the cell · 2009Article
- Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells.Journal of cell science · 2008Article
- Intermediate filaments: a role in epithelial polarity.Experimental cell research · 2007Review
- GCP6 binds to intermediate filaments: a novel function of keratins in the organization of microtubules in epithelial cells.Molecular biology of the cell · 2007Article
- Distinct lipid rafts in subdomains from human placental apical syncytiotrophoblast membranes.The Journal of membrane biologyArticle
- Multiple roles for keratin intermediate filaments in the regulation of epithelial barrier function and apico-basal polarity.Tissue barriersReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Ezrin connects the apical F-actin scaffold to membrane proteins in the apical brush border of intestinal epithelial cells. Yet, the mechanisms that recruit ezrin to the apical domain remain obscure. Using stable CACO-2 transfectants expressing keratin 8 (K8) antisense RNA under a tetracycline-responsive element, we showed that the actin-ezrin scaffold cannot assemble in the absence of intermediate filaments (IFs). Overexpression of ezrin partially rescued this phenotype. Overexpression of K8 in mice also disrupted the assembly of the brush border, but ezrin distributed away from the apical membrane in spots along supernumerary IFs. In cytochalasin D-treated cells ezrin localized to a subapical compartment and coimmunoprecipitated with IFs. Overexpression of ezrin in undifferentiated cells showed a Triton-insoluble ezrin compartment negative for phospho-T567 (dormant) ezrin visualized as spots along IFs. Pulse-chase analysis showed that Triton-insoluble, newly synthesized ezrin transiently coimmunoprecipitates with IFs during the first 30 min of the chase. Dormant, but not active (p-T567), ezrin bound in vitro to isolated denatured keratins in Far-Western analysis and to native IFs in pull-down assays. We conclude that a transient association to IFs is an early step in the polarized assembly of apical ezrin in intestinal epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.