ArticleAnnals of clinical microbiology and antimicrobials2005
Development of a Multiple-Locus Variable Number of Tandem Repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia.
Article in Annals of clinical microbiology and antimicrobials, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 65 citations in OpenAlex.
- A Retrospective Cohort Study of Leptospirosis in Crete, Greece.Tropical medicine and infectious disease · 2025Article
- The serological and genetic diversity of theJournal of veterinary research · 2023Article
- Unique Features of Tandem Repeats in Bacteria.Journal of bacteriology · 2020Article
- Assessment of disinfectant and antibiotic susceptibility patterns and multi-locus variable number tandem repeat analysis ofIranian journal of microbiology · 2018Article
- Comparison of virulence gene profiles and genomic fingerprints ofTropical medicine and health · 2018Article
- High-Resolution Melting Curve Analysis of the 16S Ribosomal Gene to Detect and Identify Pathogenic and SaprophyticThe American journal of tropical medicine and hygiene · 2017Article
- Profiling of Leptospira interrogans, L. santarosai, L. meyeri and L. borgpetersenii by SE-AFLP, PFGE and susceptibility testing--a continuous attempt at species and serovar differentiation.Emerging microbes & infections · 2016Article
- Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis.Jundishapur journal of microbiology · 2015Article
- A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains.Journal of medical microbiology · 2015Article
- Isolation of Leptospira interrogans serovar Hardjoprajitno from a calf with clinical leptospirosis in Chile.BMC veterinary research · 2015Article
- Leptospirosis in humans.Current topics in microbiology and immunology · 2015Review
- Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina.Memorias do Instituto Oswaldo Cruz · 2014Article
- Epizootiological survey of small mammals as Leptospira spp. reservoirs in Eastern Croatia.Acta tropica · 2014Article
- High-resolution typing of Leptospira interrogans strains by multispacer sequence typing.Journal of clinical microbiology · 2014Article
- Molecular typing of Leptospira spp. strains isolated from field mice confirms a link to human leptospirosis.Epidemiology and infection · 2013Article
- Detection of virulence factors and molecular typing of pathogenic Leptospira from capybara (Hydrochaeris hydrochaeris).Current microbiology · 2012Article
- Application and validation of PFGE for serovar identification of Leptospira clinical isolates.PLoS neglected tropical diseases · 2010Article
- Monitoring Leptospira strain collections: the need for quality control.The American journal of tropical medicine and hygiene · 2010Article
- Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.Journal of medical microbiology · 2009Article
- Variable nucleotide tandem-repeat analysis revealing a unique group of Leptospira interrogans serovar Pomona isolates associated with California sea lions.Journal of clinical microbiology · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeptospirosis is a zoonotic disease caused by the genus, Leptospira. Leptospira interrogans is the most common genomospecies implicated in the disease. Epidemiological investigations are needed to distinguish outbreak situations or to trace reservoirs of the organisms. Current methodologies used for typing Leptospira have significant drawbacks. The development of an easy to perform yet high resolution method is needed for this organism.
methodsIn this study we have searched the available genomic sequence of L. interrogans serovar Copenhageni strain Fiocruz L1-130 for the presence of tandem repeats. These repeats were evaluated against reference strains for diversity. Six loci were selected to create a Multiple Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) to explore the genetic diversity within L. interrogans serovar Australis clinical isolates from Far North Queensland.
resultsThe 39 reference strains used for the development of the method displayed 39 distinct patterns. Diversity Indexes for the loci varied between 0.80 and 0.93 and the number of repeat units at each locus varied between less than one to 52 repeats. When the MLVA was applied to serovar Australis isolates three large clusters were distinguishable, each comprising various hosts including Rattus species, human and canines.
conclusionThe MLVA described in this report, was easy to perform, analyse and was reproducible. The loci selected had high diversity allowing discrimination between serovars and also between strains within a serovar. This method provides a starting point on which improvements to the method and comparisons to other techniques can be made.
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