ArticleProceedings of the National Academy of Sciences of the United States of America2005
Nonmetabolizable analogue of 2-oxoglutarate elicits heterocyst differentiation under repressive conditions in Anabaena sp. PCC 7120.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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61 citing papers in PubMed, 136 citations in OpenAlex.
- Coordination of NProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Co-option of an ancestral peptidase controls developmental patterning in multicellular cyanobacteria.iScience · 2026Article
- Hacking heterocysts: advances in the genetic regulation of heterocyst differentiation.Archives of microbiology · 2025Review
- Carbon metabolism shapes FtsZ levels and cell division in the cyanobacterium Anabaena PCC 7120.Communications biology · 2025Article
- Analysing the Cyanobacterial PipX Interaction Network Using NanoBiT Complementation inInternational journal of molecular sciences · 2024Article
- ppGpp accumulation reduces the expression of the global nitrogen homeostasis-modulating NtcA regulon by affecting 2-oxoglutarate levels.Communications biology · 2023Article
- A c-di-GMP binding effector controls cell size in a cyanobacterium.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Effects of glutamate oxaloacetate transaminase on reactive oxygen species in Ganoderma lucidum.Applied microbiology and biotechnology · 2023Article
- Low nitrogen stress-induced transcriptome changes revealed the molecular response and tolerance characteristics in maintaining the C/N balance of sugar beet (Frontiers in plant science · 2023Article
- c-di-GMP Homeostasis Is Critical for Heterocyst Development inFrontiers in microbiology · 2021Article
- Inactivation of Three RG(S/T)GR Pentapeptide-Containing Negative Regulators of HetR Results in Lethal Differentiation ofLife (Basel, Switzerland) · 2020Article
- HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacteriumeLife · 2020Article
- Carbon/nitrogen homeostasis control in cyanobacteria.FEMS microbiology reviews · 2020Review
- Article
- Preventing Accidental Heterocyst Development in Cyanobacteria.Journal of bacteriology · 2019Article
- cyAbrB Transcriptional Regulators as Safety Devices To Inhibit Heterocyst Differentiation inJournal of bacteriology · 2019Article
- The Pkn22 Kinase ofFrontiers in microbiology · 2019Article
- Biosensors-Based In Vivo Quantification of 2-Oxoglutarate in Cyanobacteria and Proteobacteria.Life (Basel, Switzerland) · 2018Article
- Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria.Plant physiology · 2018Article
- Coordinating carbon and nitrogen metabolic signaling through the cyanobacterial global repressor NdhR.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
1 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In response to combined nitrogen starvation in the growth medium, the filamentous cyanobacterium Anabaena sp. PCC 7120 is able to develop a particular cell type, called a heterocyst, specialized in molecular nitrogen fixation. Heterocysts are regularly intercalated among vegetative cells and represent 5-10% of all cells along each filament. In unicellular cyanobacteria, the key Krebs cycle intermediate, 2-oxoglutarate (2-OG), has been suggested as a nitrogen status signal, but in vivo evidence is still lacking. In this study we show that nitrogen starvation causes 2-OG to accumulate transiently within cells of Anabaena PCC 7120, reaching a maximal intracellular concentration of approximately 0.1 mM 1 h after combined nitrogen starvation. A nonmetabolizable fluorinated 2-OG derivative, 2,2-difluoropentanedioic acid (DFPA), was synthesized and used to demonstrate the signaling function of 2-OG in vivo. DFPA is shown to be a structural analogue of 2-OG and the process of its uptake and accumulation in vivo can be followed by (19)F magic angle spinning NMR because of the presence of the fluorine atom and its chemical stability. DFPA at a threshold concentration of 0.3 mM triggers heterocyst differentiation under repressing conditions. The multidisciplinary approaches using synthetic fluorinated analogues, magic angle spinning NMR for their analysis in vivo, and techniques of molecular biology provide a powerful means to identify the nature of the signals that remain unknown or poorly defined in many signaling pathways.
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