ArticleBMC molecular biology2005
Cloning and characterization of cDNAs encoding putative CTCFs in the mosquitoes, Aedes aegypti and Anopheles gambiae.
Article in BMC molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Chromatin Structure and Function in Mosquitoes.Frontiers in genetics · 2020Review
- Suppressor of hairy-wing, modifier of mdg4 and centrosomal protein of 190 gene orthologues of the gypsy insulator complex in the malaria mosquito, Anopheles stephensi.Insect molecular biology · 2016Article
- Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster.BMC biology · 2015Article
- Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.Insects · 2015Review
- Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Conserved boundary elements from the Hox complex of mosquito, Anopheles gambiae.Nucleic acids research · 2013Article
- The chromatin insulator CTCF and the emergence of metazoan diversity.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Chromatin domain boundary element search tool for Drosophila.Nucleic acids research · 2012Article
- Insulators as mediators of intra- and inter-chromosomal interactions: a common evolutionary theme.Journal of biology · 2009Review
- Loss of the insulator protein CTCF during nematode evolution.BMC molecular biology · 2009Article
- Vertebrate Protein CTCF and its Multiple Roles in a Large-Scale Regulation of Genome Activity.Current genomics · 2009Article
- Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis.BMC molecular biology · 2007Article
- Genomic mapping of Suppressor of Hairy-wing binding sites in Drosophila.Genome biology · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundOne of the many ascribed functions of CCCTC-binding factor (CTCF) in vertebrates is insulation of genes via enhancer-blocking. Insulation allows genes to be shielded from "cross-talk" with neighboring regulatory elements. As such, endogenous insulator sequences would be valuable elements to enable stable transgene expression. Recently, CTCF joined Su(Hw), Zw5, BEAF32 and GAGA factor as a protein associated with insulator activity in the fruitfly, Drosophila melanogaster. To date, no known insulators have been described in mosquitoes.
resultsWe have identified and characterized putative CTCF homologs in the medically-important mosquitoes, Aedes aegypti and Anopheles gambiae. These genes encode polypeptides with eleven C2H2 zinc fingers that show significant similarity to those of vertebrate CTCFs, despite at least 500 million years of divergence. The mosquito CTCFs are constitutively expressed and are upregulated in early embryos and in the ovaries of blood-fed females. We have uncovered significant bioinformatics evidence that CTCF is widespread, at least among Drosophila species. Finally, we show that the An. gambiae CTCF binds two known insulator sequences.
conclusionMosquito CTCFs are likely orthologous to the widely-characterized vertebrate CTCFs and potentially also serve an insulating function. As such, CTCF may provide a powerful tool for improving transgene expression in these mosquitoes through the identification of endogenous binding sites.
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