ArticleJournal of neuro-oncology2005
Gene expression profile induced by BCNU in human glioma cell lines with differential MGMT expression.
Article in Journal of neuro-oncology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Extracting the Strongest Signals from Omics Data: Differentially Expressed Pathways and Beyond.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Magnetic nanoparticles for tumor imaging and therapy: a so-called theranostic system.Pharmaceutical research · 2013Review
- Changes in mouse cognition and hippocampal gene expression observed in a mild physical- and blast-traumatic brain injury.Neurobiology of disease · 2013Article
- BTECH: a platform to integrate genomic, transcriptomic and epigenomic alterations in brain tumors.Neuroinformatics · 2011Article
- Direct DNA methylation profiling using methyl binding domain proteins.Analytical chemistry · 2010Article
- Gene expression profiles of 1-(4-amino-2-methyl-5-pyrimidinyl)-methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU)-resistant C6 rat glioma cells.Journal of neuro-oncology · 2006Article
- Mechanisms of malignant glioma immune resistance and sources of immunosuppression.Gene therapy & molecular biology · 2006Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemotherapy with the alkylating agent BCNU (1,3-bis (2-chloroethyl)-1-nitrosourea) is the most commonly used chemotherapeutic agent for gliomas. However, the usefulness of this agent is limited because tumor cell resistance to BCNU is frequently found in clinical brain tumor therapy. The O6-methylguanine-DNA methyltransferase protein (MGMT) reverses alkylation at the O6 position of guanine and we have reported the role of MGMT in the response of brain tumors to alkylating agents. However, the different mechanisms underlying the patterns related to MGMT remain unclear. To better understand the molecular mechanism by which BCNU exerts its effect in glioma cell lines according MGMT expression, we used microarray technology to interrogate 3800 known genes and determine the gene expression profiles altered by BCNU treatment. Our results showed that treatment with BCNU alters the expression of a diverse group of genes in a time-dependent manner. A subset of gene changes was found common in both glioma cell lines and other subset is specific of each cell line. After 24 h of BCNU treatment, up-regulation of transcription factors involved in the nucleation of both RNA polymerase II and III transcription initiation complexes was reported. Interestingly, BCNU promoted the expression of actin-dependent regulators of chromatin. Similar effects were found with higher BCNU doses in MGMT+ cell line showing a similar mechanism that in MGMT-deficient cell with standard doses. Our data suggest that human glioma cell lines treated with BCNU, independently of MGMT expression, show changes in the expression of cell cycle and survival-related genes interfering the transcription mechanisms and the chromatin regulation.
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