ArticleThe Journal of biological chemistry2005
Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy.
Article in The Journal of biological chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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113 citing papers in PubMed, 173 citations in OpenAlex.
- Article
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- SELEX based aptamers with diagnostic and entry inhibitor therapeutic potential for SARS-CoV-2.Scientific reports · 2023Article
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- Nano-Enabled Antivirals for Overcoming Antibody Escaped Mutations Based SARS-CoV-2 Waves.International journal of molecular sciences · 2023Review
- A Protein Co-Conservation Network Model Characterizes Mutation Effects on SARS-CoV-2 Spike Protein.International journal of molecular sciences · 2023Article
- Emerging Dominant SARS-CoV-2 Variants.Journal of chemical information and modeling · 2023Article
- Bionics design of affinity peptide inhibitors for SARS-CoV-2 RBD to block SARS-CoV-2 RBD-ACE2 interactions.Heliyon · 2023Article
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- Persistent Laplacian projected Omicron BA.4 and BA.5 to become new dominating variants.Computers in biology and medicine · 2022Article
- Emerging dominant SARS-CoV-2 variants.ArXiv · 2022Article
- Cross-species recognition and molecular basis of SARS-CoV-2 and SARS-CoV binding to ACE2s of marine animals.National science review · 2022Article
- Development of antibody resistance in emerging mutant strains of SARS CoV-2: Impediment for COVID-19 vaccines.Reviews in medical virology · 2022Review
- PEDV: Insights and Advances into Types, Function, Structure, and Receptor Recognition.Viruses · 2022Review
- Mathematical artificial intelligence design of mutation-proof COVID-19 monoclonal antibodies.ArXiv · 2022Article
- Structures of a deltacoronavirus spike protein bound to porcine and human receptors.Nature communications · 2022Article
- Emerging Vaccine-Breakthrough SARS-CoV-2 Variants.ACS infectious diseases · 2022Article
- Omicron Variant (B.1.1.529): Infectivity, Vaccine Breakthrough, and Antibody Resistance.Journal of chemical information and modeling · 2022Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
19 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) is a recently identified human coronavirus. The extremely high homology of the viral genomic sequences between the viruses isolated from human (huSARS-CoV) and those of palm civet origin (pcSARS-CoV) suggested possible palm civet-to-human transmission. Genetic analysis revealed that the spike (S) protein of pcSARS-CoV and huSARS-CoV was subjected to the strongest positive selection pressure during transmission, and there were six amino acid residues within the receptor-binding domain of the S protein being potentially important for SARS progression and tropism. Using the single-round infection assay, we found that a two-amino acid substitution (N479K/T487S) of a huSARS-CoV for those of pcSARS-CoV almost abolished its infection of human cells expressing the SARS-CoV receptor ACE2 but no effect upon the infection of mouse ACE2 cells. Although single substitution of these two residues had no effects on the infectivity of huSARS-CoV, these recombinant S proteins bound to human ACE2 with different levels of reduced affinity, and the two-amino acid-substituted S protein showed extremely low affinity. On the contrary, substitution of these two amino acid residues of pcSARS-CoV for those of huSRAS-CoV made pcSARS-CoV capable of infecting human ACE2-expressing cells. These results suggest that amino acid residues at position 479 and 487 of the S protein are important determinants for SARS-CoV tropism and animal-to-human transmission.
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