ArticleAntimicrobial agents and chemotherapy2005
Sixteen homologs of the mex-type multidrug resistance efflux pump in Bacteroides fragilis.
Article in Antimicrobial agents and chemotherapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 44 citations in OpenAlex.
- Antimicrobial resistance is widespread among intestinal and extra-intestinalInfection and immunity · 2025Article
- Advances in drug resistance and resistance mechanisms of four colorectal cancer-associated gut microbiota.PeerJ · 2025Review
- Disentangling hindgut metabolism in the American cockroach through single-cell genomics and metatranscriptomics.Frontiers in microbiology · 2023Article
- Antimicrobial susceptibility profiles and tentative epidemiological cutoff values ofFrontiers in microbiology · 2022Article
- Antibiotic resistomes discovered in the gut microbiomes of Korean swine and cattle.GigaScience · 2020Article
- Article
- Benzoate- and Salicylate-Tolerant Strains of Escherichia coli K-12 Lose Antibiotic Resistance during Laboratory Evolution.Applied and environmental microbiology · 2017Article
- Multidrug Efflux Systems in Microaerobic and Anaerobic Bacteria.Antibiotics (Basel, Switzerland) · 2015Review
- The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.Clinical microbiology reviews · 2015Review
- Analysis of the outer membrane proteome and secretome of Bacteroides fragilis reveals a multiplicity of secretion mechanisms.PloS one · 2015Article
- Mechanisms of reduced susceptibility and genotypic prediction of antibiotic resistance in Prevotella isolated from cystic fibrosis (CF) and non-CF patients.The Journal of antimicrobial chemotherapy · 2014Article
- Microbial efflux systems and inhibitors: approaches to drug discovery and the challenge of clinical implementation.The open microbiology journal · 2013Article
- Review
- Presence of quorum-sensing systems associated with multidrug resistance and biofilm formation in Bacteroides fragilis.Microbial ecology · 2008Article
- Impact of anatomic site on growth, efflux-pump expression, cell structure, and stress responsiveness of Bacteroides fragilis.Current microbiology · 2007Article
- Bacteroides: the good, the bad, and the nitty-gritty.Clinical microbiology reviews · 2007Review
- Efflux pump overexpression in multiple-antibiotic-resistant mutants of Bacteroides fragilis.Antimicrobial agents and chemotherapy · 2006Article
- Identification of a multidrug efflux pump in Flavobacterium johnsoniae.Veterinary researchArticle
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sixteen homologs of multidrug resistance efflux pump operons of the resistance-nodulation-cell division (RND) family were found in the Bacteroides fragilis genome sequence by homology searches. Disruption mutants were made to the mexB homologs of the four genes most similar to Pseudomonas aeruginosa mexB. Reverse transcription-PCR was conducted and indicated that the genes were transcribed in a polycistronic fashion and that the promoter was upstream of bmeA (the mexA homolog). One of these disruption mutants (in bmeB, the mexB homolog) was more susceptible than the parental strain to certain cephems, polypeptide antibiotics, fusidic acid, novobiocin, and puromycin. The gene for this homolog and the adjacent upstream gene, bmeA, were cloned in a hypersensitive Escherichia coli host. The resultant transformants carrying B. fragilis bmeAB were more resistant to certain agents; these agents also had lower MICs for the B. fragilis bmeB disruption mutants than for the parental strain. The putative efflux pump operon is composed of bmeA, bmeB, and bmeC (a putative outer membrane channel protein homologous with OprM). Addition of the efflux pump inhibitors, carbonyl cyanide m-chlorophenylhydrazone (a proton conductor that eliminates the energy source) and Phe-Arg beta-naphthylamide (MC-207,110) (the first specific inhibitor described for RND pumps in P. aeruginosa), resulted in lowered MICs in the parental strain but not in the bmeB disruption mutant, indicating that the bmeB pump is affected by these inhibitors. This is the first description of RND type pumps in the genus Bacteroides.
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