ArticleInvestigative ophthalmology & visual science2005
Corneal keratocytes: phenotypic and species differences in abundant protein expression and in vitro light-scattering.
Article in Investigative ophthalmology & visual science, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 116 citations in OpenAlex.
- Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates.International journal of molecular sciences · 2024Article
- Regenerative Therapy for Corneal Scarring Disorders.Biomedicines · 2024Review
- Collagen crosslinking impacts stromal wound healing and haze formation in a rabbit phototherapeutic keratectomy model.Molecular vision · 2023Article
- Topographic Outcomes in Keratoconus Surgery: Epi-on versus Epi-off Iontophoresis Corneal Collagen Cross-Linking.Journal of clinical medicine · 2022Article
- Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy.Cells · 2022Article
- Atypical Cellular Elements of Unknown Origin in the Subbasal Nerve Plexus of a Diabetic Cornea Diagnosed by Large-Area Confocal Laser Scanning Microscopy.Diagnostics (Basel, Switzerland) · 2021Article
- Article
- Fibroblastic and bone marrow-derived cellularity in the corneal stroma.Experimental eye research · 2021Article
- Effect of porcine corneal stromal extract on keratocytes from SMILE-derived lenticules.Journal of cellular and molecular medicine · 2021Article
- Modulation of human corneal stromal cell differentiation by hepatocyte growth factor and substratum compliance.Experimental eye research · 2018Article
- Corneal chemical burn treatment through a delivery system consisting of TGF-βDrug delivery and translational research · 2018Article
- Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy.Journal of cellular and molecular medicine · 2018Article
- Latrunculin B and substratum stiffness regulate corneal fibroblast to myofibroblast transformation.Experimental eye research · 2018Article
- Tissue and cellular biomechanics during corneal wound injury and repair.Acta biomaterialia · 2017Article
- Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts.Scientific reports · 2017Article
- Article
- Antimicrobial Blue Light Therapy for Infectious Keratitis: Ex Vivo and In Vivo Studies.Investigative ophthalmology & visual science · 2017Article
- Electrospun Scaffolds for Corneal Tissue Engineering: A Review.Materials (Basel, Switzerland) · 2016Review
- Three-Dimensional Construction of a Rabbit Anterior Corneal Replacement for Lamellar Keratoplasty.PloS one · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposePrevious studies suggest that corneal haze after injury involves changes in the light-scattering properties of keratocytes that are possibly linked to the abundant expression of water-soluble proteins. The purpose of this study was to determine the protein expression pattern of keratocytes from different species and different cultured rabbit keratocyte phenotypes and to assess differences in light-scattering in vitro.
methodsWater-soluble proteins were isolated from corneal epithelial cells and keratocytes of several species, including human (Hu), mouse (Mo), rabbit (Ra), chicken (Ch), and pig (P) and different cultured rabbit keratocyte phenotypes. Proteins were then characterized by SDS-PAGE, tryptic peptide sequence analysis, and Western blot analysis. Light-scattering and actin organization from cultured cells were determined with confocal reflectance and fluorescence microscopy, respectively.
resultsProtein expression patterns varied substantially between species and cell types, with five new abundantly expressed proteins identified including, LDH (Ra, Ch), G3PDH (Hu, Ch), pyruvate kinase (Ch), Annexin II (Ch), and protein disulfide isomerase (Ch). Different rabbit keratocyte phenotypes also showed different levels of expression of ALDH1A1 and TKT, with myofibroblasts showing the greatest reduction. Myofibroblasts showed significantly greater (P < 0.05) light-scattering but also showed the greatest organization of actin filaments.
conclusionsAbundant protein expression is a characteristic feature of corneal keratocytes that is lost when cells are phenotypically modulated in culture. Greater light-scattering by myofibroblasts also provides support for a link between cellular transparency and haze after injury that is possibly related to loss of protein expression or development of prominent actin filament bundles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.