ArticlePlant physiology2005
Water uptake and distribution in germinating tobacco seeds investigated in vivo by nuclear magnetic resonance imaging.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 220 citations in OpenAlex.
- Insights into Salinity Stress-Induced Morpho-Physiological and Molecular Responses and Nanoparticle- and Nanobiochar-Mediated Tolerance Mechanisms During Seed Germination.Nanomaterials (Basel, Switzerland) · 2026Review
- Water-Holding Capacity, Ion Release, and Saturation Dynamics of Mosses as Micro-Scale Buffers Against Water Stress in Semi-Arid Ecosystems.Plants (Basel, Switzerland) · 2025Article
- Development History, Structure, and Function ofInternational journal of molecular sciences · 2024Review
- Spatial specificity of metabolism regulation of abscisic acid-imposed seed germination inhibition in Korean pine (Pinus koraiensis sieb et zucc).Frontiers in plant science · 2024Article
- Water phase distribution and its dependence on internal structure in soaking maize kernels: a study using low-field nuclear magnetic resonance and X-ray micro-computed tomography.Frontiers in plant science · 2024Article
- Effect of Abiotic Stresses from Drought, Temperature, and Density on Germination and Seedling Growth of Barley (Plants (Basel, Switzerland) · 2023Article
- Red LED Light Improves Pepper (International journal of molecular sciences · 2023Article
- Exogenous gibberellic acid shortening after-ripening process and promoting seed germination in a medicinal plant Panax notoginseng.BMC plant biology · 2023Article
- Characterization and mechanism of seed dormancy inFrontiers in plant science · 2023Article
- The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds.Frontiers in plant science · 2023Article
- Identification of a DEAD-box RNA Helicase BnRH6 Reveals Its Involvement in Salt Stress Response in Rapeseed (International journal of molecular sciences · 2022Article
- Cell Membrane Features as Potential Breeding Targets to Improve Cold Germination Ability of Seeds.Plants (Basel, Switzerland) · 2022Review
- Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination inInternational journal of molecular sciences · 2022Article
- Tailored Media Are Key to Unlocking the Diversity of Endophytic Bacteria in Distinct Compartments of Germinating Seeds.Microbiology spectrum · 2022Article
- Underlying Biochemical and Molecular Mechanisms for Seed Germination.International journal of molecular sciences · 2022Review
- Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography.Plant direct · 2022Article
- Abscisic Acid: Role in Fruit Development and Ripening.Frontiers in plant science · 2022Review
- Study on pecan seed germination influenced by seed endocarp.Open life sciences · 2022Article
- The Opposite Roles of White Light in Regulating Germination of Fresh and Aged Seed in Tobacco.Plants (Basel, Switzerland) · 2021Article
- Acceleration in GerminationPlants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of water uptake of germinating tobacco (Nicotiana tabacum) seeds was studied spatially and temporally by in vivo (1)H-nuclear magnetic resonance (NMR) microimaging and (1)H-magic angle spinning NMR spectroscopy. These nondestructive state-of-the-art methods showed that water distribution in the water uptake phases II and III is inhomogeneous. The micropylar seed end is the major entry point of water. The micropylar endosperm and the radicle show the highest hydration. Germination of tobacco follows a distinct pattern of events: rupture of the testa is followed by rupture of the endosperm. Abscisic acid (ABA) specifically inhibits endosperm rupture and phase III water uptake, but does not alter the spatial and temporal pattern of phase I and II water uptake. Testa rupture was associated with an increase in water uptake due to initial embryo elongation, which was not inhibited by ABA. Overexpression of beta-1,3-glucanase in the seed-covering layers of transgenic tobacco seeds did not alter the moisture sorption isotherms or the spatial pattern of water uptake during imbibition, but partially reverted the ABA inhibition of phase III water uptake and of endosperm rupture. In vivo (13)C-magic angle spinning NMR spectroscopy showed that seed oil mobilization is not inhibited by ABA. ABA therefore does not inhibit germination by preventing oil mobilization or by decreasing the water-holding capacity of the micropylar endosperm and the radicle. Our results support the proposal that different seed tissues and organs hydrate at different extents and that the micropylar endosperm region of tobacco acts as a water reservoir for the embryo.
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