ArticleBiophysical journal2005
CD8 kinetically promotes ligand binding to the T-cell antigen receptor.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- The Combination of CD8αα and Peptide-MHC-I in a Face-to-Face Mode Promotes Chicken γδT Cells Response.Frontiers in immunology · 2020Article
- Membrane Organization and Physical Regulation of Lymphocyte Antigen Receptors: A Biophysicist's Perspective.The Journal of membrane biology · 2019Review
- Extent of MHC Clustering Regulates Selectivity and Effectiveness of T Cell Responses.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins.Frontiers in molecular biosciences · 2019Review
- CD8Immunology and cell biology · 2017Article
- Dynamic quantification of antigen molecules with flow cytometry.Journal of immunological methods · 2015Article
- To affinity and beyond: harnessing the T cell receptor for cancer immunotherapy.Human vaccines & immunotherapeutics · 2014Review
- A mathematical model for the coreceptors SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE3 in BRASSINOSTEROID INSENSITIVE1-mediated signaling.Plant physiology · 2013Article
- Why must T cells be cross-reactive?Nature reviews. Immunology · 2012Review
- Review
- Evidence that the density of self peptide-MHC ligands regulates T-cell receptor signaling.PloS one · 2012Article
- Insights into T cell recognition of antigen: significance of two-dimensional kinetic parameters.Frontiers in immunology · 2012Article
- Mechanisms controlling granule-mediated cytolytic activity of cytotoxic T lymphocytes.Immunologic research · 2011Review
- Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Interleukin-15:Interleukin-15 receptor α scaffold for creation of multivalent targeted immune molecules.Protein engineering, design & selection : PEDS · 2011Article
- Article
- Article
- CD8 controls T cell cross-reactivity.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism of CD8 cooperation with the TCR in antigen recognition was studied on live T cells. Fluorescence correlation measurements yielded evidence of the presence of two TCR and CD8 subpopulations with different lateral diffusion rate constants. Independently, evidence for two subpopulations was derived from the experimentally observed two distinct association phases of cognate peptide bound to class I MHC (pMHC) tetramers and the T cells. The fast phase rate constant ((1.7 +/- 0.2) x 10(5) M(-1) s(-1)) was independent of examined cell type or MHC-bound peptides' structure. Its value was much faster than that of the association of soluble pMHC and TCR ((7.0 +/- 0.3) x 10(3) M(-1) s(-1)), and close to that of the association of soluble pMHC with CD8 ((1-2) x 10(5) M(-1) s(-1)). The fast binding phase disappeared when CD8-pMHC interaction was blocked by a CD8-specific mAb. The latter rate constant was slowed down approximately 10-fold after cells treatment with methyl-beta-cyclodextrin. These results suggest that the most efficient pMHC-cell association route corresponds to a fast tetramer binding to a colocalized CD8-TCR subpopulation, which apparently resides within membrane rafts: the reaction starts by pMHC association with the CD8. This markedly faster step significantly increases the probability of pMHC-TCR encounters and thereby promotes pMHC association with CD8-proximal TCR. The slow binding phase is assigned to pMHC association with a noncolocalized CD8-TCR subpopulation. Taken together with results of cytotoxicity assays, our data suggest that the colocalized, raft-associated CD8-TCR subpopulation is the one capable of inducing T-cell activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.