ArticleJournal of the Society for Gynecologic Investigation2005
Gestational-dependent changes in the expression of signal transduction and contractile filament-associated proteins in mouse myometrium.
Article in Journal of the Society for Gynecologic Investigation, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 15 citations in OpenAlex.
- Effect of estrogen on molecular and functional characteristics of the rodent vaginal muscularis.The journal of sexual medicine · 2013Article
- Phosphorylation of h1 calponin by PKC epsilon may contribute to facilitate the contraction of uterine myometrium in mice during pregnancy and labor.Reproductive biology and endocrinology : RB&E · 2012Article
- Altered contribution of RhoA/Rho kinase signaling in contractile activity of myometrium in leptin receptor-deficient mice.American journal of physiology. Endocrinology and metabolism · 2011Article
- Myometrial mechanoadaptation during pregnancy: implications for smooth muscle plasticity and remodelling.Journal of cellular and molecular medicine · 2008Article
- Towards understanding the myometrial physiome: approaches for the construction of a virtual physiological uterus.BMC pregnancy and childbirth · 2007Review
- Regulation of the uterine contractile apparatus and cytoskeleton.Seminars in cell & developmental biology · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesThe timely onset of powerful uterine contractions during parturition involves the integration of many signaling pathways, the protein components of which may be translationaly regulated throughout gestation. We have utilized the pregnant mouse model to examine gestational-dependent changes in the expression of several proteins implicated in the coordination of myometrial excitation-contraction coupling: caveolins 1-3, rho-associated kinases (ROKalpha and beta), alpha-actin, MLC(20), and h-caldesmon.
methodsProtein expression was examined by Western blotting of myometrial homogenates from nonpregnant mice (NP) and compared to that from pregnant mice on gestational days 12, 15, 17, and 19 (term = day 19).
resultsAll protein expressions were unchanged during the estrous cycle. alpha-Actin was found to be invariant throughout pregnancy. The expressions of caveolin-1, -2 and -3, when compared to alpha-actin expression, also did not change significantly with mid- to late pregnancy. h-caldesmon: alpha-actin, ROKalpha:alpha-actin, and ROKbeta:alpha-actin ratios were, however, significantly elevated on day 19, whereas MLC(20):alpha-actin was significantly down-regulated on day 12 to day 19. Consistent with elevated ROK expression at term, the ROK inhibitor Y27632 gave a greater reduction of thromboxane-stimulated contractions in myometrium from day 19 mice compared to NP mice.
conclusionsThese data suggest that in mouse myometrium there is a dynamic regulation of the expression of several proteins implicated in contractile signal integration. This may be important for regulating (1) relative uterine quiescence to ensure pregnancy progression and (2) priming the tissue for requisite contractile effort at term.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.