ArticleMolecular therapy : the journal of the American Society of Gene Therapy2005
Postmitotic nuclear retention of episomal plasmids is altered by DNA labeling and detection methods.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Mechanisms and Strategies for Enhancing DNA Nuclear Entry in Gene Delivery.Cellular and molecular bioengineering · 2026Article
- Electroporation- and Liposome-Mediated Co-Transfection of Single and Multiple Plasmids.Pharmaceutics · 2025Article
- The intracellular visualization of exogenous DNA in fluorescence microscopy.Drug delivery and translational research · 2024Review
- Nuclear Entry of DNA and Transgene Expression in Dividing and Non-dividing Cells.Cellular and molecular bioengineering · 2023Article
- DNA-induced spatial entrapment of general transcription machinery can stabilize gene expression in a nondividing cell.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cytoplasmic transport and nuclear import of plasmid DNA.Bioscience reports · 2017Review
- A Triple-Fluorophore-Labeled Nucleic Acid pH Nanosensor to Investigate Non-viral Gene Delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Identification of an alveolar type I epithelial cell-specific DNA nuclear import sequence for gene delivery.Gene therapy · 2016Article
- Single-cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Spatiotemporal cellular imaging of polymer-pDNA nanocomplexes affords in situ morphology and trafficking trends.Molecular pharmaceutics · 2013Article
- Highly acetylated tubulin permits enhanced interactions with and trafficking of plasmids along microtubules.Gene therapy · 2013Article
- Transcription factor plasmid binding modulates microtubule interactions and intracellular trafficking during gene transfer.Gene therapy · 2012Article
- DNA nuclear targeting sequences for non-viral gene delivery.Pharmaceutical research · 2011Article
- Quantitative analysis of condensation/decondensation status of pDNA in the nuclear sub-domains by QD-FRET.Nucleic acids research · 2011Article
- Cyclic stretch of alveolar epithelial cells alters cytoskeletal micromechanics.Biotechnology and bioengineering · 2011Article
- The SP-C promoter facilitates alveolar type II epithelial cell-specific plasmid nuclear import and gene expression.Gene therapy · 2010Article
- Identification of protein cofactors necessary for sequence-specific plasmid DNA nuclear import.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Cell-specific nuclear import of plasmid DNA in smooth muscle requires tissue-specific transcription factors and DNA sequences.Gene therapy · 2008Article
- Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells.Molecular biology of the cell · 2007Article
- Intracellular trafficking of plasmids for gene therapy: mechanisms of cytoplasmic movement and nuclear import.Current gene therapy · 2006Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
One often overlooked aspect of nonviral gene therapy is the maintenance and localization of plasmids within a transfected cell. In this study we have quantified the nuclear retention of plasmids within microinjected cells after a single round of cell division. We employed several commercially available reagents to label plasmids with fluorophores for our microinjection tracking experiments. Interestingly, plasmids labeled with different techniques produced drastically different results. Naked plasmids microinjected directly into nuclei and later detected by in situ hybridization were found almost exclusively within the nuclei of the daughter cells after mitosis and were partitioned between the daughter nuclei with a normal, Gaussian distribution. Identical results were obtained with plasmids labeled with a fluorescent peptide nucleic acid. However, when plasmids were labeled with several commercially available fluorescent DNA labeling kits that randomly attach fluorophores to the entire plasmid and injected into HeLa cell nuclei, the modified plasmids were excluded from daughter nuclei after cell division. Taken together, these results suggest that naked, unmodified plasmids are retained in the nucleus following cell division and likely continue to express in the daughter cells. Our results demonstrate the significant alterations in episome localization that the labeling technique itself can have on plasmid trafficking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.