ArticleMolecular biology of the cell2005
Taxol-stabilized microtubules can position the cytokinetic furrow in mammalian cells.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 71 citations in OpenAlex.
- Microtubule regulation in cancer cells.Frontiers in cell and developmental biology · 2025Article
- Involvement of RBP-J interacting and tubulin-associated protein in the distribution of protein regulator of cytokinesis 1 in mitotic spindles.Frontiers in cell and developmental biology · 2024Article
- Microtubule detyrosination by VASH1/SVBP is regulated by the conformational state of tubulin in the lattice.Current biology : CB · 2023Article
- Phytochemicals as Chemo-Preventive Agents and Signaling Molecule Modulators: Current Role in Cancer Therapeutics and Inflammation.International journal of molecular sciences · 2022Review
- Early Pharmacological Profiling of Antiproliferative Compounds by Live Cell Imaging.Molecules (Basel, Switzerland) · 2022Article
- Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells.Life science alliance · 2021Article
- Comparative analysis of taxol-derived fluorescent probes to assess microtubule networks in a complex live three-dimensional tissue.Cytoskeleton (Hoboken, N.J.) · 2020Article
- Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1.Journal of cell science · 2019Article
- Site occupancy calibration of taxane pharmacology in live cells and tissues.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A.eLife · 2018Article
- Atypical Cadherin Dachsous1b Interacts with Ttc28 and Aurora B to Control Microtubule Dynamics in Embryonic Cleavages.Developmental cell · 2018Article
- Two-step interphase microtubule disassembly aids spindle morphogenesis.BMC biology · 2018Article
- Anaphase Chromosomes in Crane-Fly Spermatocytes Treated With Taxol (Paclitaxel) Accelerate When Their Kinetochore Microtubules Are Cut: Evidence for Spindle Matrix Involvement With Spindle Forces.Frontiers in cell and developmental biology · 2018Article
- Is inflammatory micronucleation the key to a successful anti-mitotic cancer drug?Open biology · 2017Review
- The MgcRacGAP SxIP motif tethers Centralspindlin to microtubule plus ends inJournal of cell science · 2017Article
- The RNA-binding protein ATX-2 regulates cytokinesis through PAR-5 and ZEN-4.Molecular biology of the cell · 2016Article
- Permeabilization activated reduction in fluorescence: A novel method to measure kinetics of protein interactions with intracellular structures.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Synthesis and anticancer activity of all known (-)-agelastatin alkaloids.The Journal of organic chemistry · 2013Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
How microtubules act to position the plane of cell division during cytokinesis is a topic of much debate. Recently, we showed that a subpopulation of stable microtubules extends past chromosomes and interacts with the cell cortex at the site of furrowing, suggesting that these stabilized microtubules may stimulate contractility. To test the hypothesis that stable microtubules can position furrows, we used taxol to rapidly suppress microtubule dynamics during various stages of mitosis in PtK1 cells. Cells with stabilized prometaphase or metaphase microtubule arrays were able to initiate furrowing when induced into anaphase by inhibition of the spindle checkpoint. In these cells, few microtubules contacted the cortex. Furrows formed later than usual, were often aberrant, and did not progress to completion. Images showed that furrowing correlated with the presence of one or a few stable spindle microtubule plus ends at the cortex. Actin, myosin II, and anillin were all concentrated in these furrows, demonstrating that components of the contractile ring can be localized by stable microtubules. Inner centromere protein (INCENP) was not found in these ingressions, confirming that INCENP is dispensable for furrow positioning. Taxol-stabilization of the numerous microtubule-cortex interactions after anaphase onset delayed furrow initiation but did not perturb furrow positioning. We conclude that taxol-stabilized microtubules can act to position the furrow and that loss of microtubule dynamics delays the timing of furrow onset and prevents completion. We discuss our findings relative to models for cleavage stimulation.
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