ArticleNucleic acids research2005
PCNA acts as a stationary loading platform for transiently interacting Okazaki fragment maturation proteins.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
- Spatial organization and dynamics of genome replication: from forks to foci.Nucleic acids research · 2026Review
- PARP1-mediated 5' flap dynamics facilitate Okazaki fragment maturation.bioRxiv : the preprint server for biology · 2026Article
- JMJD1B-mediated FEN1 demethylation allows timely switching of Okazaki fragment maturation core enzymes to avoid mutagenic flap ligation by PARP1-LIG3.bioRxiv : the preprint server for biology · 2025Article
- Dynamic association of H3K36me3 with pericentromeric heterochromatin regulates its replication time.EMBO reports · 2025Article
- Histone variant macroH2A1 regulates synchronous firing of replication origins in the inactive X chromosome.Nucleic acids research · 2024Article
- A FRET-Based Assay for the Identification of PCNA Inhibitors.International journal of molecular sciences · 2023Article
- Proliferating cell nuclear antigen inhibitors block distinct stages of herpes simplex virus infection.PLoS pathogens · 2023Article
- The dynamical organization of the core pluripotency transcription factors responds to differentiation cues in early S-phase.Frontiers in cell and developmental biology · 2023Article
- Post-Translational Modifications of PCNA: Guiding for the Best DNA Damage Tolerance Choice.Journal of fungi (Basel, Switzerland) · 2022Review
- Cytosine base modifications regulate DNA duplex stability and metabolism.Nucleic acids research · 2021Article
- Review
- Developmental differences in genome replication program and origin activation.Nucleic acids research · 2020Article
- Are the processes of DNA replication and DNA repair reading a common structural chromatin unit?Nucleus (Austin, Tex.) · 2020Review
- Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals.Nature communications · 2020Article
- Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.Nucleus (Austin, Tex.) · 2019Article
- DNA replication dynamics of vole genome and its epigenetic regulation.Epigenetics & chromatin · 2019Article
- Peripheral re-localization of constitutive heterochromatin advances its replication timing and impairs maintenance of silencing marks.Nucleic acids research · 2018Article
- Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex.Nucleic acids research · 2018Article
- Simultaneous dual-channel imaging to quantify interdependent protein recruitment to laser-induced DNA damage sites.Nucleus (Austin, Tex.) · 2018Article
- A novel non-canonical PIP-box mediates PARG interaction with PCNA.Nucleic acids research · 2017Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In DNA replication, the leading strand is synthesized continuously, but lagging strand synthesis requires the complex, discontinuous synthesis of Okazaki fragments, and their subsequent joining. We have used a combination of in situ extraction and dual color photobleaching to compare the dynamic properties of three proteins essential for lagging strand synthesis: the polymerase clamp proliferating cell nuclear antigen (PCNA) and two proteins that bind to it, DNA Ligase I and Fen1. All three proteins are localized at replication foci (RF), but in contrast to PCNA, Ligase and Fen1 were readily extracted. Dual photobleaching combined with time overlays revealed a rapid exchange of Ligase and Fen1 at RF, which is consistent with de novo loading at every Okazaki fragment, while the slow recovery of PCNA mostly occurred at adjacent, newly assembled RF. These data indicate that PCNA works as a stationary loading platform that is reused for multiple Okazaki fragments, while PCNA binding proteins only transiently associate and are not stable components of the replication machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.