ArticleGenetics2005
Minos as a genetic and genomic tool in Drosophila melanogaster.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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Who cites it
107 citing papers in PubMed.
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- LKRSDH-dependent histone modifications of insulin-like peptide sites contribute to age-related circadian rhythm changes.Nature communications · 2024Article
- The Drosophila ZNRF1/2 homologue, detour, interacts with HOPS complex and regulates autophagy.Communications biology · 2024Article
- Genome-wide association in Drosophila identifies a role for Piezo and Proc-R in sleep latency.Scientific reports · 2024Article
- Threat gates visual aversion via theta activity in Tachykinergic neurons.Nature communications · 2023Article
- Insulin-mediated endothelin signaling is antiviral during West Nile virus infection.bioRxiv : the preprint server for biology · 2023Article
- Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature.Bioresources and bioprocessing · 2022Article
- Identification of theCells · 2022Article
- An expanded toolkit foreLife · 2022Article
- Knockdown of a Cyclic Nucleotide-Gated Ion Channel Impairs Locomotor Activity and Recovery From Hypoxia in AdultFrontiers in physiology · 2022Article
- Slo2/KThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Identification of Genes Contributing to a Long Circadian Period inJournal of biological rhythms · 2021Article
- Physical and Chemical Barriers in the Larval Midgut Confer Developmental Resistance to Virus Infection inViruses · 2021Article
- Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.PLoS genetics · 2021Article
- Intruder (DD38E), a recently evolved sibling family of DD34E/Tc1 transposons in animals.Mobile DNA · 2020Article
- A genetic screen in Drosophila reveals an unexpected role for the KIP1 ubiquitination-promoting complex in male fertility.PLoS genetics · 2020Article
47 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Much of the information about the function of D. melanogaster genes has come from P-element mutagenesis. The major drawback of the P element, however, is its strong bias for insertion into some genes (hotspots) and against insertion into others (coldspots). Within genes, 5'-UTRs are preferential targets. For the successful completion of the Drosophila Genome Disruption Project, the use of transposon vectors other than P will be necessary. We examined here the suitability of the Minos element from Drosophila hydei as a tool for Drosophila genomics. Previous work has shown that Minos, a member of the Tc1/mariner family of transposable elements, is active in diverse organisms and cultured cells; it produces stable integrants in the germ line of several insect species, in the mouse, and in human cells. We generated and analyzed 96 Minos integrations into the Drosophila genome and devised an efficient "jump-starting" scheme for production of single insertions. The ratio of insertions into genes vs. intergenic DNA is consistent with a random distribution. Within genes, there is a statistically significant preference for insertion into introns rather than into exons. About 30% of all insertions were in introns and approximately 55% of insertions were into or next to genes that have so far not been hit by the P element. The insertion sites exhibit, in contrast to other transposons, little sequence requirement beyond the TA dinucleotide insertion target. We further demonstrate that induced remobilization of Minos insertions can delete nearby sequences. Our results suggest that Minos is a useful tool complementing the P element for insertional mutagenesis and genomic analysis in Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.