ArticleExtremophiles : life under extreme conditions2005
Gene replacement in Haloarcula marismortui: construction of a strain with two of its three chromosomal rRNA operons deleted.
Article in Extremophiles : life under extreme conditions, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Two types of sheathed archaellum structures from Haloarcula marismortui differ in their outer layer architectures.Nature communications · 2026Article
- Rft1 catalyzes lipid-linked oligosaccharide translocation across the ER membrane.Nature communications · 2024Article
- The dark side of the ribosome life cycle.RNA biology · 2022Article
- Effect of single-residue bulges on RNA double-helical structures: crystallographic database analysis and molecular dynamics simulation studies.Journal of molecular modeling · 2017Article
- Expression and Function of Different Guanine-Plus-Cytosine Content 16S rRNA Genes inFrontiers in microbiology · 2017Article
- An Acidic Exopolysaccharide fromArchaea (Vancouver, B.C.) · 2017Article
- Overview of the genetic tools in the Archaea.Frontiers in microbiology · 2012Article
- Utilization of vinasse for the production of polyhydroxybutyrate by Haloarcula marismortui.Folia microbiologica · 2012Article
- Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium.Extremophiles : life under extreme conditions · 2009Article
- Mutations outside the anisomycin-binding site can make ribosomes drug-resistant.Journal of molecular biology · 2008Article
- Genetic and biochemical characterization of the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthase in Haloferax mediterranei.Journal of bacteriology · 2008Article
- Intragenomic 16S rDNA divergence in Haloarcula marismortui is an adaptation to different temperatures.Journal of molecular evolution · 2007Article
- Selection for intragenic suppressors of lethal 23S rRNA mutations in Escherichia coli identifies residues important for ribosome assembly and function.Molecular genetics and genomics : MGG · 2007Article
- Molecular characterization of the phaECHm genes, required for biosynthesis of poly(3-hydroxybutyrate) in the extremely halophilic archaeon Haloarcula marismortui.Applied and environmental microbiology · 2007Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Site-directed mutagenesis were done in Haloarcula marismortui using the strategy that Khorana and coworkers devised for deleting the bacteriorhodopsin gene from Halobacterium halobium [Krebs et al. Proc Natl Acad Sci USA 90:1987-1991 (1993)]. Strains have been prepared from H. marsimortui, which normally has three rRNA operons, that are missing either its rrnB operon or both its rrnB and rrnC operons. In rich media, both strains grow at about the same rate as wild type. The G2099 in the 23S rRNA gene of the single operon strain was changed to A, and a three amino acid deletion was introduced into the gene for ribosomal protein L22 of the wild-type organism. The structural consequences of these and other such mutations can be determined with unusual accuracy because crystals of the large ribosomal subunit of H. marismortui diffract to atomic resolution.
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