ArticleThe Journal of biological chemistry2005
Myristic acid stimulates endothelial nitric-oxide synthase in a CD36- and an AMP kinase-dependent manner.
Article in The Journal of biological chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- The multifaceted roles of fatty acids and their dysregulation in obese mothers: potential implications for infant development.Nutrition & metabolism · 2025Review
- Insight into the mechanism of lipids binding and uptake by CD36 receptor.Bioinformation · 2015Article
- Thrombospondin-1 and CD47 regulation of cardiac, pulmonary and vascular responses in health and disease.Matrix biology : journal of the International Society for Matrix Biology · 2014Review
- Article
- Thrombospondin-1 inhibits VEGF receptor-2 signaling by disrupting its association with CD47.The Journal of biological chemistry · 2010Article
- Metabolomic profiling of cellular responses to carvedilol enantiomers in vascular smooth muscle cells.PloS one · 2010Article
- The role of thrombospondins in wound healing, ischemia, and the foreign body reaction.Journal of cell communication and signaling · 2009Article
- Molecular regulation of tumor angiogenesis and perfusion via redox signaling.Chemical reviews · 2009Review
- Regulation of nitric oxide signalling by thrombospondin 1: implications for anti-angiogenic therapies.Nature reviews. Cancer · 2009Review
- Cellular fatty acid uptake: a pathway under construction.Trends in endocrinology and metabolism: TEM · 2009Review
- Enhancing cardiovascular dynamics by inhibition of thrombospondin-1/CD47 signaling.Current drug targets · 2008Review
- Thrombospondin-1 inhibits nitric oxide signaling via CD36 by inhibiting myristic acid uptake.The Journal of biological chemistry · 2007Article
- CD36 may determine our desire for dietary fats.The Journal of clinical investigation · 2005Review
Corrections and comments
- Retraction · 2013-03-01Falsification/Fabrication of Image · Investigation by Company/Institution · Investigation by ORI · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author · · NIH finding of Misconduct for Eric J Smart:
- Commented on by
- Commented on by
- Retracted
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Dietary free fatty acids have been reported to have various effects on the endothelium including the generation of nitric oxide. The goal of the current study was to determine the mechanism whereby free fatty acid causes an increase in nitric oxide synthesis. The specific hypothesis tested was that free fatty acid association with CD36, a class B scavenger receptor, induces the activation of endothelial nitric-oxide synthase (eNOS). A human microvascular endothelial cell line and a transfected Chinese hamster ovary cell system were used to determine which free fatty acids stimulate eNOS. Surprisingly, only myristic acid, and to a lesser extent palmitic acid, stimulated eNOS. The stimulation of eNOS was dose- and time-dependent. Competition experiments with other free fatty acids and with a CD36-blocking antibody demonstrated that the effects of myristic acid on eNOS required association with CD36. Further mechanistic studies demonstrated that the effects of myristic acid on eNOS function were not dependent on PI 3-kinase, Akt kinase, or calcium. Pharmacological studies and dominant negative constructs were used to demonstrate that myristic acid/CD36 stimulation of eNOS activity was dependent on the activation of AMP kinase. These data demonstrate an unexpected link among myristic acid, CD36, AMP kinase, and eNOS activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.