ArticleJournal of bacteriology2005
Architecture and adhesive activity of the Haemophilus influenzae Hsf adhesin.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- Review
- Substitutions in the nonactive site of the passenger domain on the activity ofInfection and immunity · 2024Article
- Article
- Utilization of Variant and Fusion Proteins To Functionally Map the Aggregatibacter actinomycetemcomitans Trimeric Autotransporter Protein ApiA.Infection and immunity · 2018Article
- Bioinformatic analysis of meningococcal Msf and Opc to inform vaccine antigen design.PloS one · 2018Article
- The crystal structure of PD1, a Haemophilus surface fibril domain.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Structural Basis for Toughness and Flexibility in the C-terminal Passenger Domain of an Acinetobacter Trimeric Autotransporter Adhesin.The Journal of biological chemistry · 2016Article
- Identification and therapeutic potential of a vitronectin binding region of meningococcal msf.PloS one · 2015Article
- A fine-tuned interaction between trimeric autotransporter haemophilus surface fibrils and vitronectin leads to serum resistance and adherence to respiratory epithelial cells.Infection and immunity · 2014Article
- The Haemophilus cryptic genospecies Cha adhesin has at least two variants that differ in host cell binding, bacterial aggregation, and biofilm formation properties.Journal of bacteriology · 2014Article
- The biology of Neisseria adhesins.Biology · 2013Article
- Characterization and functional analysis of AatB, a novel autotransporter adhesin and virulence factor of avian pathogenic Escherichia coli.Infection and immunity · 2013Article
- Type V secretion: mechanism(s) of autotransport through the bacterial outer membrane.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Crystallization and X-ray diffraction analysis of a novel surface-adhesin protein: protein E from Haemophilus influenzae.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- A naturally occurring single-residue mutation in the translocator domain of Neisseria meningitidis NhhA affects trimerization, surface localization, and adhesive capabilities.Infection and immunity · 2011Article
- SadA, a trimeric autotransporter from Salmonella enterica serovar Typhimurium, can promote biofilm formation and provides limited protection against infection.Infection and immunity · 2011Article
- Structure and function of the Haemophilus influenzae autotransporters.Frontiers in cellular and infection microbiology · 2011Review
- Expression of Xylella fastidiosa fimbrial and afimbrial proteins during biofilm formation.Applied and environmental microbiology · 2010Article
- A localized multimeric anchor attaches the Caulobacter holdfast to the cell pole.Molecular microbiology · 2010Article
- Transcriptome analysis of the phytobacterium Xylella fastidiosa growing under xylem-based chemical conditions.Journal of biomedicine & biotechnology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Haemophilus influenzae type b is an important cause of meningitis and other serious invasive diseases and initiates infection by colonizing the upper respiratory tract. Among the major adhesins in H. influenzae type b is a nonpilus protein called Hsf, a large protein that forms fiber-like structures on the bacterial surface and shares significant sequence similarity with the nontypeable H. influenzae Hia autotransporter. In the present study, we characterized the structure and adhesive activity of Hsf. Analysis of the predicted amino acid sequence of Hsf revealed three regions with high-level homology to the HiaBD1 and HiaBD2 binding domains in Hia. Based on examination of glutathione S-transferase fusion proteins corresponding to these regions, two of the three had adhesive activity and one was nonadhesive in assays with cultured epithelial cells. Structural modeling demonstrated that only the two regions with adhesive activity harbored an acidic binding pocket like the binding pocket identified in the crystal structure of HiaBD1. Consistent with these results, disruption of the acidic binding pockets in the adhesive regions eliminated adhesive activity. These studies advance our understanding of the architecture of Hsf and the family of trimeric autotransporters and provide insight into the structural determinants of H. influenzae type b adherence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.