ArticleJournal of bacteriology2005
Transcriptional analysis of the conserved ftsZ gene cluster in Mycoplasma genitalium and Mycoplasma pneumoniae.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Structural basis for transcriptional regulation by the cell division regulator MraZ in Mycoplasma genitalium.Nature communications · 2026Article
- Toward the Complete Functional Characterization of a Minimal Bacterial Proteome.The journal of physical chemistry. B · 2022Article
- The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock.Molecular biology reports · 2022Article
- Functional Characterization of the Cell Division Gene Cluster of the Wall-less BacteriumFrontiers in microbiology · 2021Article
- Potential Molecular Targets for Narrow-Spectrum Agents to Combat Mycoplasma pneumoniae Infection and Disease.Frontiers in microbiology · 2016Review
- Intrinsic terminators in Mycoplasma hyopneumoniae transcription.BMC genomics · 2015Article
- Article
- Inhibition of cell division induced by external guide sequences (EGS Technology) targeting ftsZ.PloS one · 2012Article
- Mycoplasma hyopneumoniae transcription unit organization: genome survey and prediction.DNA research : an international journal for rapid publication of reports on genes and genomes · 2011Article
- Absolute quantification of microbial proteomes at different states by directed mass spectrometry.Molecular systems biology · 2011Article
- Transcriptional regulation of MG_149, an osmoinducible lipoprotein gene from Mycoplasma genitalium.Molecular microbiology · 2011Article
- Transcriptional response of Mycoplasma genitalium to osmotic stress.Microbiology (Reading, England) · 2011Article
- Strand-specific deep sequencing of the transcriptome.Genome research · 2010Article
- Characterization of a unique ClpB protein of Mycoplasma pneumoniae and its impact on growth.Infection and immunity · 2008Article
- Differential expression of lipoprotein genes in Mycoplasma pneumoniae after contact with human lung epithelial cells, and under oxidative and acidic stress.BMC microbiology · 2008Article
- Lipoprotein multigene families in Mycoplasma pneumoniae.Journal of bacteriology · 2006Article
- Probing in vivo promoter activities in Mycoplasma pneumoniae: a system for generation of single-copy reporter constructs.Applied and environmental microbiology · 2006Article
- Synthesis, stability, and function of cytadhesin P1 and accessory protein B/C complex of Mycoplasma pneumoniae.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Several experimental approaches were used to construct a detailed transcriptional profile of the phylogenetically conserved ftsZ cell division gene cluster in both Mycoplasma genitalium and its closest relative, Mycoplasma pneumoniae. We determined initiation and termination points for the cluster, as well as an absolute steady-state RNA level for each gene. Transcription of this cluster in both these organisms was shown to be highly strand specific. While the four genes in this cluster are cotranscribed, their transcription unit also includes two genes of close proximity yet disparate function. A transcription initiation point immediately upstream of these two genes was detected in M. genitalium but not M. pneumoniae. In M. pneumoniae, transcription of the six genes terminates at a poly(U)-tailed hairpin. In M. genitalium, this transcription terminates at two closely spaced points by an unknown mechanism. Real-time reverse transcription-PCR analysis of this cluster in M. pneumoniae shows that mRNA levels for all six genes vary at most fivefold and form a gradient of decreasing quantity with increasing distance from the promoter at the beginning of the cluster. mRNA from coding regions was approximately 20- to 100-fold more abundant than that from intergenic regions. We estimated the most abundant mRNA we detected at 0.6 copy per cell. We conclude that groups of functionally related genes in M. genitalium and M. pneumoniae are often preceded by promoters but rarely followed by terminators. This causes functionally unrelated genes to be commonly cotranscribed in these organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.