ArticleJournal of bacteriology2005
Stable accumulation of sigma54 in Helicobacter pylori requires the novel protein HP0958.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Menadione reduces the expression of virulence- and colonization-associated genes inMicrobiology (Reading, England) · 2025Article
- A two-hybrid system reveals previously uncharacterized protein-protein interactions within the Helicobacter pylori NIF iron-sulfur maturation system.Scientific reports · 2021Article
- Identification of Potential Drug Targets inPathogens (Basel, Switzerland) · 2020Article
- Iron-sulfur protein maturation in Helicobacter pylori: identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets.Molecular microbiology · 2018Article
- Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Role of Flagella in the Pathogenesis of Helicobacter pylori.Current microbiology · 2017Review
- Hypothetical protein CT398 (CdsZ) interacts with σ(54) (RpoN)-holoenzyme and the type III secretion export apparatus in Chlamydia trachomatis.Protein science : a publication of the Protein Society · 2015Article
- Basal Body Structures Differentially Affect Transcription of RpoN- and FliA-Dependent Flagellar Genes in Helicobacter pylori.Journal of bacteriology · 2015Article
- Article
- Themes and Variations: Regulation of RpoN-Dependent Flagellar Genes across Diverse Bacterial Species.Scientifica · 2014Review
- Insertion mutations in Helicobacter pylori flhA reveal strain differences in RpoN-dependent gene expression.Microbiology (Reading, England) · 2013Article
- A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen Helicobacter pylori.Applied and environmental microbiology · 2012Article
- The zinc-ribbon domain of Helicobacter pylori HP0958: requirement for RpoN accumulation and possible roles of homologs in other bacteria.Microbial informatics and experimentation · 2011Article
- Ulcerogenic Helicobacter pylori strains isolated from children: a contribution to get insight into the virulence of the bacteria.PloS one · 2011Article
- Motility and chemotaxis in Campylobacter and Helicobacter .Annual review of microbiology · 2011Review
- Built shallow to maintain homeostasis and persistent infection: insight into the transcriptional regulatory network of the gastric human pathogen Helicobacter pylori.PLoS pathogens · 2010Review
- The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori.BMC microbiology · 2010Article
- Effect of FliK mutation on the transcriptional activity of the {sigma}54 sigma factor RpoN in Helicobacter pylori.Microbiology (Reading, England) · 2009Article
- Posttranscriptional regulation of flagellin synthesis in Helicobacter pylori by the RpoN chaperone HP0958.Journal of bacteriology · 2008Article
- Regulation of the Helicobacter pylori Fe-S cluster synthesis protein NifS by iron, oxidative stress conditions, and fur.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Several flagellar genes in Helicobacter pylori are dependent on sigma(54) (RpoN) for their expression. These genes encode components of the basal body, the hook protein, and a minor flagellin, FlaB. A protein-protein interaction map for H. pylori constructed from a high-throughput screen of a yeast two-hybrid assay (http://pim.hybrigenics.com/pimriderext/common/) revealed interactions between sigma(54) and the conserved hypothetical protein HP0958. To see if HP0958 influences sigma(54) function, the corresponding gene was disrupted with a kanamycin resistance gene (aphA3) in H. pylori ATCC 43504 and the resulting mutant was analyzed. The hp0958:aphA3 mutant was nonmotile and failed to produce flagella. Introduction of a functional copy of hp0958 into the genome of the hp0958:aphA3 mutant restored flagellar biogenesis and motility. The hp0958:aphA3 mutant was deficient in expressing two sigma(54)-dependent reporter genes, flaB'-'xylE and hp1120'-'xylE. Levels of sigma(54) in the hp0958 mutant were substantially lower than those in the parental strain, suggesting that the failure of the mutant to express the genes in the RpoN regulon and produce flagella was due to reduced sigma(54) levels. Expressing sigma(54) at high levels by putting rpoN under the control of the ureA promoter restored flagellar biogenesis and motility in the hp0958:aphA3 mutant. Turnover of sigma(54) was more rapid in the hp0958:aphA3 mutant than it was in the wild-type strain, suggesting that HP0958 supports wild-type sigma(54) levels in H. pylori by protecting it from proteolysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.