ArticleProceedings of the National Academy of Sciences of the United States of America2005
Mammalian electrophysiology on a microfluidic platform.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed.
- Integrated microfluidic biosensors: shaping the future of quantitative life sciences and on-chip molecular diagnostics.Lab on a chip · 2026Review
- Giant unilamellar vesicles as a model system for studying ion transport.Biophysical reviews · 2025Review
- Microfluidic platforms for monitoring cardiomyocyte electromechanical activity.Microsystems & nanoengineering · 2025Review
- A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy.Lab on a chip · 2024Article
- Point-of-care microfluidic devices for pathogen detection.Biosensors & bioelectronics · 2018Review
- Article
- Review
- Droplet-interface-bilayer assays in microfluidic passive networks.Scientific reports · 2015Article
- The role of tissue engineering and biomaterials in cardiac regenerative medicine.The Canadian journal of cardiology · 2014Review
- Making a hydrophoretic focuser tunable using a diaphragm.Biomicrofluidics · 2014Article
- Laminar stream of detergents for subcellular neurite damage in a microfluidic device: a simple tool for the study of neuroregeneration.Journal of neural engineering · 2013Article
- Microtechnologies to fuel neurobiological research with nanometer precision.Journal of nanobiotechnology · 2013Review
- Model systems for cardiovascular regenerative biology.Cold Spring Harbor perspectives in medicine · 2013Review
- Multi-electrode array technologies for neuroscience and cardiology.Nature nanotechnology · 2013Review
- Quantification of the specific membrane capacitance of single cells using a microfluidic device and impedance spectroscopy measurement.Biomicrofluidics · 2012Article
- Synapse microarray identification of small molecules that enhance synaptogenesis.Nature communications · 2011Article
- Whole-Cell Electrical Activity Under Direct Mechanical Stimulus by AFM Cantilever Using Planar Patch Clamp Chip Approach.Cellular and molecular bioengineering · 2011Article
- A microfluidic device for simultaneous electrical and mechanical measurements on single cells.Biomicrofluidics · 2011Article
- Microfluidics-based lab-on-chip systems in DNA-based biosensing: an overview.Sensors (Basel, Switzerland) · 2011Review
- From understanding cellular function to novel drug discovery: the role of planar patch-clamp array chip technology.Frontiers in pharmacology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The recent development of automated patch clamp technology has increased the throughput of electrophysiology but at the expense of visual access to the cells being studied. To improve visualization and the control of cell position, we have developed a simple alternative patch clamp technique based on microfluidic junctions between a main chamber and lateral recording capillaries, all fabricated by micromolding of polydimethylsiloxane (PDMS). PDMS substrates eliminate the need for vibration isolation and allow direct cell visualization and manipulation using standard microscopy. Microfluidic integration allows recording capillaries to be arrayed 20 microm apart, for a total chamber volume of <0.5 nl. The geometry of the recording capillaries permits high-quality, stable, whole-cell seals despite the hydrophobicity of the PDMS surface. Using this device, we are able to demonstrate reliable whole-cell recording of mammalian cells on an inexpensive microfluidic platform. Recordings of activation of the voltage-sensitive potassium channel Kv2.1 in mammalian cells compare well with traditional pipette recordings. The results make possible the integration of whole-cell electrophysiology with easily manufactured microfluidic lab-on-a-chip devices.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.