ArticleNucleic acids research2005
Detection of single DNA molecules by multicolor quantum-dot end-labeling.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 75 citations in OpenAlex.
- Review: immunoassays in DNA damage and instability detection.Cellular and molecular life sciences : CMLS · 2019Review
- Duplex-Specific Nuclease-Amplified Detection of MicroRNA Using Compact Quantum Dot-DNA Conjugates.ACS applied materials & interfaces · 2018Article
- Quantum dots-DNA bioconjugates: synthesis to applications.Interface focus · 2016Review
- Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices.Nucleic acids research · 2014Article
- Revisiting 30 years of biofunctionalization and surface chemistry of inorganic nanoparticles for nanomedicine.Frontiers in chemistry · 2014Review
- Assessing the stochastic intermittency of single quantum dot luminescence for robust quantification of biomolecules.Analytical chemistry · 2013Article
- Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.Chemical reviews · 2013Review
- Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine.Biochimica et biophysica acta · 2010Article
- Enzymatic digestion of single DNA molecules anchored on nanogold-modified surfaces.Nanoscale research letters · 2009Article
- Tracking of single quantum dot labeled EcoRV sliding along DNA manipulated by double optical tweezers.Biophysical journal · 2009Article
- See me, feel me: methods to concurrently visualize and manipulate single DNA molecules and associated proteins.Nucleic acids research · 2008Review
- Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA.Nucleic acids research · 2008Article
- Fluorescence in situ hybridization (FISH) on maize metaphase chromosomes with quantum dot-labeled DNA conjugates.Chromosoma · 2008Article
- Single-step multicolor fluorescence in situ hybridization using semiconductor quantum dot-DNA conjugates.Cell biochemistry and biophysics · 2006Article
- A simple DNA stretching method for fluorescence imaging of single DNA molecules.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Observation of DNA-protein interactions by single molecule fluorescence microscopy is usually performed by using fluorescent DNA binding agents. However, such dyes have been shown to induce cleavage of the DNA molecule and perturb its interactions with proteins. A new method for the detection of surface-attached DNA molecules by fluorescence microscopy is introduced in this paper. Biotin- and/or digoxigenin-modified DNA fragments are covalently linked at both extremities of a DNA molecule via sequence-specific hybridization and ligation. After the modified DNA molecules have been stretched on a glass surface, their ends are visualized by multicolor fluorescence microscopy using conjugated quantum dots (QD). We demonstrate that under carefully selected conditions, the position and orientation of individual DNA molecules can be inferred with good efficiency from the QD fluorescence signals alone. This is achieved by selecting QD pairs that have the distance and direction expected for the combed DNA molecules. Direct observation of single DNA molecules in the absence of DNA staining agent opens new possibilities in the fundamental study of DNA-protein interactions. This work also documents new possibilities regarding the use of QD for nucleic acid detection and analysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.