ArticleBritish journal of pharmacology2005
M(2) and M(3) muscarinic receptor-mediated contractions in longitudinal smooth muscle of the ileum studied with receptor knockout mice.
Article in British journal of pharmacology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 91 citations in OpenAlex.
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- Sex-dependent regulation of cholinergic responses in the mouse colon: functional evidence for reduced acetylcholinesterase-dependent control and nitrergic modulation in females.Journal of muscle research and cell motility · 2026Article
- Effects of Vonoprazan on Isolated Rat Lower Esophageal Sphincter and Ileum.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Antispasmodic activity of a series of cyclic imides (phthalimides) derivatives.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Contractions of ex-vivo mouse bladder driven by activation of channelrhodopsin-2 expressed in urothelial cells.Scientific reports · 2025Article
- Unravelling the Role of Post-Junctional M2 Muscarinic Receptors in Cholinergic Nerve-Mediated Contractions of Airway Smooth Muscle.International journal of molecular sciences · 2025Review
- Herbal Amara extract induces gastric fundus relaxation via inhibition of the M2 muscarinic receptor.Neurogastroenterology and motility · 2025Article
- Pharmacological effects of monoterpene carveol on the neuromuscular system of nematodes and mammals.Frontiers in pharmacology · 2024Article
- Novel Anthranilic Acid Hybrids-An Alternative Weapon against Inflammatory Diseases.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Possible role of transient receptor potential melastatin 4 channels in adrenergic contractions in mouse prostate smooth muscles.The Journal of veterinary medical science · 2023Article
- Effects of dibenzylbutyrolactone lignans arctigenin and trachelogenin on the motility of isolated rat ileum.Toxicology reports · 2022Article
- The effect of thymol on acetylcholine-induced contractions of the rat ileum and uterus underFrontiers in pharmacology · 2022Article
- Contribution of Postjunctional M2 Muscarinic Receptors to Cholinergic Nerve-Mediated Contractions of Murine Airway Smooth Muscle.Function (Oxford, England) · 2022Article
- Functions of Muscarinic Receptor Subtypes in Gastrointestinal Smooth Muscle: A Review of Studies with Receptor-Knockout Mice.International journal of molecular sciences · 2021Review
- The use of Euphorbia hirta L. (Euphorbiaceae) in diarrhea and constipation involves calcium antagonism and cholinergic mechanisms.BMC complementary medicine and therapies · 2020Article
- Involvement of transient receptor potential melastatin 4 channels in the resting membrane potential setting and cholinergic contractile responses in mouse detrusor and ileal smooth muscles.The Journal of veterinary medical science · 2019Article
- Possible antagonistic effects of the TRPC4 channel blocker ML204 on MThe Journal of veterinary medical science · 2018Article
- DoesVeterinary research forum : an international quarterly journal · 2017Article
- Type 3 muscarinic receptors contribute to intestinal mucosal homeostasis and clearance of Nippostrongylus brasiliensis through induction of TH2 cytokines.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Cissus sicyoides: Pharmacological Mechanisms Involved in the Anti-Inflammatory and Antidiarrheal Activities.International journal of molecular sciences · 2016Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Isometric contractile responses to carbachol were studied in ileal longitudinal smooth muscle strips from wild-type mice and mice genetically lacking M(2) or M(3) muscarinic receptors, in order to characterize the mechanisms involved in M(2) and M(3) receptor-mediated contractile responses. Single applications of carbachol (0.1-100 microM) produced concentration-dependent contractions in preparations from M(2)-knockout (KO) and M(3)-KO mice, mediated via M(3) and M(2) receptors, respectively, as judged by the sensitivity of contractile responses to blockade by the M(2)-preferring antagonist methoctramine (300 nM) or the M(3)-preferring antagonist 4-DAMP (30 nM). The M(2)-mediated contractions were mimicked in shape by submaximal stimulation with high K(+) concentrations (up to 35 mM), almost abolished by voltage-dependent Ca(2+) channel (VDCC) antagonists or depolarization with 140 mM K(+) medium, and greatly reduced by pertussis toxin (PTX) treatment. The M(3)-mediated contractions were only partially inhibited by VDCC antagonists or 140 mM K(+)-depolarization medium, and remained unaffected by PTX treatment. The contractions observed during high K(+) depolarization consisted of different components, either sensitive or insensitive to extracellular Ca(2+). The carbachol contractions observed with wild-type preparations consisted of PTX-sensitive and -insensitive components. The PTX-sensitive component was functionally significant only at low carbachol concentrations. The results suggest that the M(2) receptor, through PTX-sensitive mechanisms, induces ileal contractions that depend on voltage-dependent Ca(2+) entry, especially associated with action potential discharge, and that the M(3) receptor, through PTX-insensitive mechanisms, induces contractions that depend on voltage-dependent and -independent Ca(2+) entry and intracellular Ca(2+) release. In intact tissues coexpressing M(2) and M(3) receptors, M(2) receptor activity appears functionally relevant only when fractional receptor occupation is relatively small.
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