ArticleMolecular and cellular biology2005
Simultaneous recruitment of coactivators by Gcn4p stimulates multiple steps of transcription in vivo.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
81 citing papers in PubMed, 106 citations in OpenAlex.
- Genome-wide analysis reveals the importance of histone acetyltransferase Esa1 in transcriptional regulation during nitrogen starvation.bioRxiv : the preprint server for biology · 2026Article
- Mot1 regulation of promoter binding by TBP varies with stress and gene expression levels independently of coactivator dependence.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- SAGA subunits Spt3 and Spt8 act directly and non-redundantly with TFIID in TBP recruitment in the Gcn4 transcriptome.Nucleic acids research · 2025Article
- Histone Acetyltransferases Gcn5 and Esa1 Regulate Occupancy of RSC to Maintain Nucleosome-Depleted Regions and Promote RSC Recruitment to Coding Regions Genome-Wide inMolecular and cellular biology · 2025Article
- Controlling circuitry underlies the growth optimization of Saccharomyces cerevisiae.Metabolic engineering · 2023Article
- An integrated SAGA and TFIID PIC assembly pathway selective for poised and induced promoters.Genes & development · 2022Article
- Activation domains can decouple the mean and noise of gene expression.Cell reports · 2022Article
- Article
- The RSC complex remodels nucleosomes in transcribed coding sequences and promotes transcription in Saccharomyces cerevisiae.Genetics · 2021Article
- CRISPR/Cas System and Factors Affecting Its Precision and Efficiency.Frontiers in cell and developmental biology · 2021Review
- Capturing and Understanding the Dynamics and Heterogeneity of Gene Expression in the Living Cell.International journal of molecular sciences · 2020Review
- An improved auxin-inducible degron system preserves native protein levels and enables rapid and specific protein depletion.Genes & development · 2019Article
- Article
- Variation of gene expression in plants is influenced by gene architecture and structural properties of promoters.PloS one · 2019Article
- Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes.Genes & development · 2018Article
- Sharing the SAGA.Trends in biochemical sciences · 2017Review
- Combinatorial Gene Regulation through Kinetic Control of the Transcription Cycle.Cell systems · 2017Article
- CRISPR/Cas9 in Genome Editing and Beyond.Annual review of biochemistry · 2016Review
- Effects of three permeases on arginine utilization in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Genome-wide cooperation by HAT Gcn5, remodeler SWI/SNF, and chaperone Ydj1 in promoter nucleosome eviction and transcriptional activation.Genome research · 2016Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Transcriptional activation by Gcn4p is dependent on the coactivators SWI/SNF, SAGA, and Srb Mediator, which are recruited by Gcn4p and stimulate assembly of the pre-initiation complex (PIC) at the ARG1 promoter in vivo. We show that recruitment of all three coactivators is nearly simultaneous with binding of Gcn4p at ARG1 and is followed quickly by PIC formation and elongation by RNA polymerase II (Pol II) through the open reading frame. Despite the simultaneous recruitment of coactivators, rapid recruitment of SWI/SNF depends on the histone acetyltransferase (HAT) subunit of SAGA (Gcn5p), a non-HAT function of SAGA, and on Mediator. SAGA recruitment in turn is strongly stimulated by Mediator and the RSC complex. Recruitment of Mediator, by contrast, occurs independently of the other coactivators at ARG1. We confirm the roles of Mediator and SAGA in TATA binding protein (TBP) recruitment and demonstrate that all four coactivators under study enhance Pol II recruitment or promoter clearance following TBP binding. We also present evidence that SWI/SNF and SAGA stimulate transcription elongation downstream from the promoter. These functions can be limited to discrete time intervals, providing evidence for multiple stages in the induction process. Our findings reveal a program of coactivator recruitment and PIC assembly that distinguishes Gcn4p from other yeast activators studied thus far.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.