ArticleThe EMBO journal2005
A Rab21/LIM-only/CH-LIM complex regulates phagocytosis via both activating and inhibitory mechanisms.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- The Regulatory Mechanism of Rab21 in Human Diseases.Molecular neurobiology · 2023Review
- Profiling cellular diversity in sponges informs animal cell type and nervous system evolution.Science (New York, N.Y.) · 2021Article
- Rab GTPases take centre stage in understandingSmall GTPases · 2020Review
- EhRab21 associates with the Golgi apparatus in Entamoeba histolytica.Parasitology research · 2020Article
- Phagocytosis by the Retinal Pigment Epithelium: Recognition, Resolution, Recycling.Frontiers in immunology · 2020Review
- DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development.BMC biology · 2019Article
- Function of small GTPases in Dictyostelium macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Regulation of early endosomes across eukaryotes: Evolution and functional homology of Vps9 proteins.Traffic (Copenhagen, Denmark) · 2018Article
- The ancient small GTPase Rab21 functions in intermediate endocytic steps in trypanosomes.Eukaryotic cell · 2014Article
- Olive (Olea europaea) leaf extract induces apoptosis and monocyte/macrophage differentiation in human chronic myelogenous leukemia K562 cells: insight into the underlying mechanism.Oxidative medicine and cellular longevity · 2014Article
- Phosphorylation of chemoattractant receptors regulates chemotaxis, actin reorganization and signal relay.Journal of cell science · 2013Article
- Evolution of Tre-2/Bub2/Cdc16 (TBC) Rab GTPase-activating proteins.Molecular biology of the cell · 2013Article
- Antagonistic control of lysosomal fusion by Rab14 and the Lyst-related protein LvsB.Traffic (Copenhagen, Denmark) · 2013Article
- The balance in the delivery of ER components and the vacuolar proton pump to the phagosome depends on myosin IK in Dictyostelium.Molecular & cellular proteomics : MCP · 2012Article
- A genome-wide over-expression screen identifies genes involved in phagocytosis in the human protozoan parasite, Entamoeba histolytica.PloS one · 2012Article
- TOR complex 2 (TORC2) in Dictyostelium suppresses phagocytic nutrient capture independently of TORC1-mediated nutrient sensing.Journal of cell science · 2012Article
- Contribution of the caspase gene sequence diversification to the specifically antiviral defense in invertebrate.PloS one · 2011Article
- Single and multiple CH (calponin homology) domain containing multidomain proteins in Dictyostelium discoideum: an inventory.Molecular biology reports · 2010Article
- Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth.EMBO reports · 2009Article
- Dynamic changes in the spatiotemporal localization of Rab21 in live RAW264 cells during macropinocytosis.PloS one · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We have identified two LIM domain proteins, LimF and ChLim, from Dictyostelium that interact with each other and with the small, Rab5-related, Rab21 GTPase to collectively regulate phagocytosis. To investigate in vivo functions, we generated cell lines that lack or overexpress LimF and ChLim and strains that express activating or inhibiting variants of Rab21. Overexpression of LimF, loss of ChLim, or expression of constitutively active Rab21 increases the rate of phagocytosis above that of wild type. Conversely, loss of LimF, overexpression of ChLim, or expression of a dominant-negative Rab21 inhibits phagocytosis. Our studies using cells carrying multiple mutations in these genes further indicate that ChLim antagonizes the activating function of Rab21-GTP during phagocytosis; in turn, LimF is required for Rab21-GTP function. Finally, we demonstrate that ChLim and LimF localize to the phagocytic cup and phago-lysosomal vesicles. We suggest that LimF, ChLim, and activated Rab21-GTP participate as a novel signaling complex that regulates phagocytic activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.