ArticleNucleic acids research2005
Monitoring tat peptide binding to TAR RNA by solid-state 31P-19F REDOR NMR.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 66 citations in OpenAlex.
- DNP-Enhanced Magic Angle Spinning Solid-State NMR Spectroscopy to Determine RNA-Ligand Interactions.Journal of the American Chemical Society · 2026Article
- Advances in solid-state NMR methods for studying RNA structures and dynamics.Magnetic resonance letters · 2025Review
- Three Decades of REDOR in Protein Science: A Solid-State NMR Technique for Distance Measurement and Spectral Editing.International journal of molecular sciences · 2023Review
- From Angstroms to Nanometers: Measuring Interatomic Distances by Solid-State NMR.Chemical reviews · 2022Review
- Incorporation and Utility of a Responsive Ribonucleoside Analogue in Probing the Conformation of a Viral RNA Motif by Fluorescence andChembiochem : a European journal of chemical biology · 2022Article
- Nucleic acid-protein interfaces studied by MAS solid-state NMR spectroscopy.Journal of structural biology: X · 2022Article
- Strategies for RNA Resonance Assignment byFrontiers in molecular biosciences · 2021Review
- AromaticAngewandte Chemie (International ed. in English) · 2020Article
- High-Sensitivity Detection of NanometerThe journal of physical chemistry. B · 2019Article
- Isotope labeling for studying RNA by solid-state NMR spectroscopy.Journal of biomolecular NMR · 2018Review
- Investigation of higher-order RNA G-quadruplex structures in vitro and in living cells byNature protocols · 2018Article
- Studying DNA G-Quadruplex Aptamer byACS omega · 2017Article
- Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy.Nucleic acids research · 2017Article
- Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.Quarterly reviews of biophysics · 2017Review
- Shortening the HIV-1 TAR RNA Bulge by a Single Nucleotide Preserves Motional Modes over a Broad Range of Time Scales.Biochemistry · 2016Article
- (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy.Journal of biomolecular NMR · 2016Article
- Magic angle spinning NMR of viruses.Progress in nuclear magnetic resonance spectroscopy · 2015Review
- Interactions of protein side chains with RNA defined with REDOR solid state NMR.Journal of biomolecular NMR · 2011Article
- 13C/15N-19F intermolecular REDOR NMR study of the interaction of TAR RNA with Tat peptides.Journal of the American Chemical Society · 2010Article
- Intermediate rate atomic trajectories of RNA by solid-state NMR spectroscopy.Journal of the American Chemical Society · 2010Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
Complexes of the HIV transactivation response element (TAR) RNA with the viral regulatory protein tat are of special interest due in particular to the plasticity of the RNA at this binding site and to the potential for therapeutic targeting of the interaction. We performed REDOR solid-state NMR experiments on lyophilized samples of a 29 nt HIV-1 TAR construct to measure conformational changes in the tat-binding site concomitant with binding of a short peptide comprising the residues of the tat basic binding domain. Peptide binding was observed to produce a nearly 4 A decrease in the separation between phosphorothioate and 2'F labels incorporated at A27 in the upper helix and U23 in the bulge, respectively, consistent with distance changes observed in previous solution NMR studies, and with models showing significant rearrangement in position of bulge residue U23 in the bound-form RNA. In addition to providing long-range constraints on free TAR and the TAR-tat complex, these results suggest that in RNAs known to undergo large deformations upon ligand binding, 31P-19F REDOR measurements can also serve as an assay for complex formation in solid-state samples. To our knowledge, these experiments provide the first example of a solid-state NMR distance measurement in an RNA-peptide complex.
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