ArticleMolecular biology of the cell2005
Targeting of Tsc13p to nucleus-vacuole junctions: a role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 80 citations in OpenAlex.
- Functional diversification across yeast lineages of the nucleus-vacuole junction forming protein Nvj1.bioRxiv : the preprint server for biology · 2026Article
- Cvm1 and its paralogue Cvm2 function as a complex at vacuolar membrane contact sites.Molecular biology of the cell · 2025Article
- Pex30-dependent membrane contact sites maintain ER lipid homeostasis.The Journal of cell biology · 2025Article
- Partitioning into ER membrane microdomains impacts autophagic protein turnover during cellular aging.Scientific reports · 2024Article
- Lysosomal membrane contact sites: Integrative hubs for cellular communication and homeostasis.Current topics in membranes · 2024Review
- A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace.International journal of molecular sciences · 2023Review
- Review
- Article
- Mechanistic dissection of macro- and micronucleophagy.Autophagy · 2021Article
- Nucleophagy-Implications for Microautophagy and Health.International journal of molecular sciences · 2020Review
- Review
- Genomic Instability and Cellular Senescence: Lessons From the Budding Yeast.Frontiers in cell and developmental biology · 2020Review
- Mechanistic insight into the nucleus-vacuole junction based on the Vac8p-Nvj1p crystal structure.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Discovery and Roles of ER-Endolysosomal Contact Sites in Disease.Advances in experimental medicine and biology · 2017Review
- Nuclear autophagy: An evolutionarily conserved mechanism of nuclear degradation in the cytoplasm.Autophagy · 2016Review
- The Malleable Nature of the Budding Yeast Nuclear Envelope: Flares, Fusion, and Fenestrations.Journal of cellular physiology · 2016Review
- Organelle remodeling at membrane contact sites.Journal of structural biology · 2016Review
- Yeast Vps13 promotes mitochondrial function and is localized at membrane contact sites.Molecular biology of the cell · 2016Article
- Mdm1/Snx13 is a novel ER-endolysosomal interorganelle tethering protein.The Journal of cell biology · 2015Article
- The yeast sphingolipid signaling landscape.Chemistry and physics of lipids · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
TSC13 is required for the biosynthesis of very-long-chain fatty acids (VLCFAs) in yeast. Tsc13p is a polytopic endoplasmic reticulum (ER) membrane protein that accumulates at nucleus-vacuole (NV) junctions, which are formed through Velcro-like interactions between Nvj1p in the perinuclear ER and Vac8p on the vacuole membrane. NV junctions mediate piecemeal microautophagy of the nucleus (PMN), during which bleb-like portions of the nucleus are extruded into invaginations of the vacuole membrane and degraded in the vacuole lumen. We report that Tsc13p is sequestered into NV junctions from the peripheral ER through Vac8p-independent interactions with Nvj1p. During nutrient limitation, Tsc13p is incorporated into PMN vesicles in an Nvj1p-dependent manner. The lumenal diameters of PMN blebs and vesicles are significantly reduced in tsc13-1 and tsc13-1 elo3-Delta mutant cells. PMN structures are also smaller in cells treated with cerulenin, an inhibitor of de novo fatty acid synthesis and elongation. The targeting of Tsc13p-GFP into NV junctions is perturbed by cerulenin, suggesting that its binding to Nvj1p depends on the availability of fatty acid substrates. These results indicate that Nvj1p retains and compartmentalizes Tsc13p at NV junctions and that VLCFAs contribute to the normal biogenesis of trilaminar PMN structures in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.