ArticleGlycobiology2005
Gastrointestinal mucins of Fut2-null mice lack terminal fucosylation without affecting colonization by Candida albicans.
Article in Glycobiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Genome-wide association meta-analysis yields 20 loci associated with gallstone disease.Nature communications · 2018Pooled it
- Senescence-associated loss of intestinal α1,2-fucose disrupts a modifiable host-microbiome homeostasis axis in people with HIV.bioRxiv : the preprint server for biology · 2026Article
- Nutrient acquisition strategies by gut microbes.Cell host & microbe · 2024Review
- Antenatal Ureaplasma infection induces ovine small intestinal goblet cell defects: a strong link with NEC pathology.Tissue barriers · 2023Article
- Genome-wide association study of chronic sputum production implicates loci involved in mucus production and infection.The European respiratory journal · 2023Article
- Finding the sweet spot: glycosylation mediated regulation of intestinal inflammation.Mucosal immunology · 2022Review
- Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine.Communications biology · 2021Article
- Gut microbiota-mediated lysophosphatidylcholine generation promotes colitis in intestinal epithelium-specific Fut2 deficiency.Journal of biomedical science · 2021Article
- Intestinal Goblet Cell Loss during Chorioamnionitis in Fetal Lambs: Mechanistic Insights and Postnatal Implications.International journal of molecular sciences · 2021Article
- Deficiency of Intestinal α1-2-Fucosylation Exacerbates Ethanol-Induced Liver Disease in Mice.Alcoholism, clinical and experimental research · 2020Article
- The intestinal microbiota in the pathogenesis of inflammatory bowel diseases: new insights into complex disease.Clinical science (London, England : 1979) · 2018Review
- Article
- Immune-driven alterations in mucin sulphation is an important mediator of Trichuris muris helminth expulsion.PLoS pathogens · 2017Article
- Intestinal fucose as a mediator of host-microbe symbiosis.Journal of immunology (Baltimore, Md. : 1950) · 2015Review
- Roles and regulation of the mucus barrier in the gut.Tissue barriers · 2015Article
- Reprograming of gut microbiome energy metabolism by the FUT2 Crohn's disease risk polymorphism.The ISME journal · 2014Article
- High-fat diet alters the oligosaccharide chains of colon mucins in mice.Histochemistry and cell biology · 2014Article
- γδ T-cell-deficient mice show alterations in mucin expression, glycosylation, and goblet cells but maintain an intact mucus layer.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Genetically dictated change in host mucus carbohydrate landscape exerts a diet-dependent effect on the gut microbiota.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Mucin-type O-glycans and their roles in intestinal homeostasis.Glycobiology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Posttranslational modification of apomucins by the sequential action of glycosyltransferases is required to produce mature mucins. The Secretor gene (FUT2) encodes an alpha(1,2)fucosyltransferase (EC 2.4.1.69) that catalyzes addition of terminal alpha(1,2)fucose residues on mucins and other molecules in mucosal epithelium. Mutant mice containing targeted replacement of Fut2 with the bacterial reporter gene lacZ were studied to determine the affect of the loss of Fut2 on glycosylation of mucins in the gastrointestinal tract. By whole organ X-gal staining, lacZ activity is prominently expressed in the foveolar pit and chief cells of the glandular stomach, Brunner's glands of the duodenum, and goblet cells in the large intestine of Fut2-LacZ-null mice. Staining with Aleuria aurantia agglutinin demonstrates loss of L-fucosylated epithelial glycans throughout the gastrointestinal tract of Fut2-LacZ-null mice, however, histologic appearance of the tissues appears normal. Analysis of oligosaccharides released from insoluble colonic mucins, largely Muc2, by mass spectrometry shows complete lack of terminal fucosylation of O-linked oligosaccharides in Fut2-LacZ-null mice. Precursor glycans accumulate with no evidence of compensation by other fucosyltransferases or sialyltransferases on mucin glycosylation. Because Candida albicans has been reported to adhere to intestinal mucins creating a potential reservoir associated with vaginitis, Fut2-LacZ-null and wild-type mice were inoculated by gastric lavage with C. albicans. We observe no difference in colonization between genotypes suggesting mucin terminal fucosylation does not significantly influence C. albicans-host interaction in the intestine, highlighting that infections caused by the same organism at different mucosal surfaces are not equal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.