ArticleClinical and experimental immunology2005
Anti-inflammatory properties of heat shock protein 70 and butyrate on Salmonella-induced interleukin-8 secretion in enterocyte-like Caco-2 cells.
Article in Clinical and experimental immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Effect of Butyrate on Food-Grade Titanium Dioxide Toxicity in Different Intestinal In Vitro Models.Chemical research in toxicology · 2024Article
- Sophy β-Glucan from the Black YeastAntioxidants (Basel, Switzerland) · 2023Article
- Avenanthramide Improves Colonic Damage Induced by Food Allergies in Mice through Altering Gut Microbiota and Regulating Hsp70-NF-κB Signaling.Nutrients · 2023Article
- Tributyrin alleviates gut microbiota dysbiosis to repair intestinal damage in antibiotic-treated mice.PloS one · 2023Article
- A narrative review of the moderating effects and repercussion of exercise intervention on osteoporosis: ingenious involvement of gut microbiota and its metabolites.Journal of translational medicine · 2022Review
- Intra- and inter-breed variation in immune response to acute and sub-chronic Salmonella infection and commercial immune-stimulant in two-layer breeds.Veterinary medicine and science · 2021Article
- Butyrate and the Fine-Tuning of Colonic Homeostasis: Implication for Inflammatory Bowel Diseases.International journal of molecular sciences · 2021Review
- Impact of Bacterial Metabolites on Gut Barrier Function and Host Immunity: A Focus on Bacterial Metabolism and Its Relevance for Intestinal Inflammation.Frontiers in immunology · 2021Review
- Crohn's Disease Variants of Nod2 Are Stabilized by the Critical Contact Region of Hsp70.Biochemistry · 2017Article
- Diet, microorganisms and their metabolites, and colon cancer.Nature reviews. Gastroenterology & hepatology · 2016Review
- Article
- The effect of NOD2 on the microbiota in Crohn's disease.Current opinion in biotechnology · 2016Review
- Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats.Journal of Zhejiang University. Science. B · 2015Article
- Salmonella enterica Serovar Typhimurium Exploits Inflammation to Modify Swine Intestinal Microbiota.Frontiers in cellular and infection microbiology · 2015Article
- Diet, microbiome, and the intestinal epithelium: an essential triumvirate?BioMed research international · 2013Review
- Carbohydrate Elimination or Adaptation Diet for Symptoms of Intestinal Discomfort in IBD: Rationales for "Gibsons' Conundrum".International journal of inflammation · 2012Article
- Microbial products from probiotic bacteria inhibit Salmonella enteritidis 857-induced IL-8 synthesis in Caco-2 cells.Folia microbiologica · 2010Article
- The protective potency of probiotic bacteria and their microbial products against enteric infections-review.Folia microbiologica · 2008Review
- Butyrate regulates the expression of pathogen-triggered IL-8 in intestinal epithelia.Pediatric research · 2007Article
- Interference of Salmonella enteritidis and Lactobacillus spp. with IL-8 levels and transepithelial electrical resistance of enterocyte-like Caco-2 cells.Folia microbiologica · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intestinal epithelial cells secrete the chemokine interleukin (IL)-8 in the course of inflammation. Because heat shock proteins (Hsps) and butyrate confer protection to enterocytes, we investigated whether they modulate Salmonella enterica serovar Enteritidis (S. serovar Enteritidis)-induced secretion of IL-8 in enterocyte-like Caco-2 cells. Caco-2 cells incubated with or without butyrate (0-20 m M, 48 h) were infected with S. serovar Enteritidis after (1 h at 42 degrees C, 6 h at 37 degrees C) or without prior heat shock (37 degrees C). Levels of Hsp70 production and IL-8 secretion were analysed using immunostaining of Western blots and enzyme-linked immunosorbent assay (ELISA), respectively. The cells secreted IL-8 in response to S. serovar Enteritidis and produced Hsp70 after heat shock or incubation with butyrate. The IL-8 secretion was inhibited by heat shock and butyrate concentrations as low as 0.2 m M for crypt-like and 1 m M for villous-like cells. In a dose-dependent manner, higher butyrate concentrations enhanced IL-8 secretion to maximal levels followed by a gradual but stable decline. This decline was associated with increasing production of Hsp70 and was more vivid in crypt-like cells. In addition, the higher concentrations abolished the heat shock inhibitory effect. Instead, they promoted the IL-8 production in heat-shocked cells even in the absence of S. serovar Enteritidis. We conclude that heat shock and low concentrations of butyrate inhibit IL-8 production by Caco-2 cells exposed to S. serovar Enteritidis. Higher butyrate concentrations stimulate the chemokine production and override the inhibitory effect of the heat shock. The IL-8 down-regulation could in part be mediated via production of Hsp70.
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