ArticleGenetics2005
RecD plays an essential function during growth at low temperature in the antarctic bacterium Pseudomonas syringae Lz4W.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- A type II toxin-antitoxin system is responsible for the cell death at low temperature in Pseudomonas syringae Lz4W lacking RNase R.The Journal of biological chemistry · 2024Article
- RNase R vs. PNPase: selecting the best-suited exoribonuclease for environmental adaptation.Extremophiles : life under extreme conditions · 2024Review
- Role of DEAD-box RNA helicases in low-temperature adapted growth of AntarcticMicrobiology spectrum · 2024Article
- Functional activity of E. coli RNase R in the Antarctic Pseudomonas syringae Lz4W.Journal, genetic engineering & biotechnology · 2023Article
- Development and Properties ofVaccines · 2022Article
- Characterization of highly conserved G-quadruplex motifs as potential drug targets in Streptococcus pneumoniae.Scientific reports · 2019Article
- Biochemical characterization of RecBCD enzyme from an Antarctic Pseudomonas species and identification of its cognate Chi (χ) sequence.PloS one · 2018Article
- Draft Genome Sequence of the Antarctic Psychrophilic Bacterium Pseudomonas syringae Strain Lz4W.Genome announcements · 2013Article
- rnr gene from the antarctic bacterium Pseudomonas syringae Lz4W, encoding a psychrophilic RNase R.Applied and environmental microbiology · 2011Article
- Biofilm formation by and thermal niche and virulence characteristics of Escherichia spp.Applied and environmental microbiology · 2011Article
- Increase in oxidative stress at low temperature in an antarctic bacterium.Current microbiology · 2011Article
- Role of the FeoB protein and siderophore in promoting virulence of Xanthomonas oryzae pv. oryzae on rice.Journal of bacteriology · 2010Article
- All three subunits of RecBCD enzyme are essential for DNA repair and low-temperature growth in the Antarctic Pseudomonas syringae Lz4W.PloS one · 2010Article
- The evolution of RecD outside of the RecBCD complex.Journal of molecular evolution · 2009Article
- Importance of trmE for growth of the psychrophile Pseudomonas syringae at low temperatures.Applied and environmental microbiology · 2009Article
- The Exiguobacterium genus: biodiversity and biogeography.Extremophiles : life under extreme conditions · 2009Article
- RecBCD enzyme and the repair of double-stranded DNA breaks.Microbiology and molecular biology reviews : MMBR · 2008Review
- Mechanism of bacterial adaptation to low temperature.Journal of biosciences · 2006Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W has been used as a model system to identify genes that are required for growth at low temperature. Transposon mutagenesis was carried out to isolate mutant(s) of the bacterium that are defective for growth at 4 degrees but normal at 22 degrees . In one such cold-sensitive mutant (CS1), the transposon-disrupted gene was identified to be a homolog of the recD gene of several bacteria. Trans-complementation and freshly targeted gene disruption studies reconfirmed that the inactivation of the recD gene leads to a cold-sensitive phenotype. We cloned, sequenced, and analyzed approximately 11.2 kbp of DNA from recD and its flanking region from the bacterium. recD was the last gene of a putative recCBD operon. The RecD ORF was 694 amino acids long and 40% identical (52% similar) to the Escherichia coli protein, and it could complement the E. coli recD mutation. The recD gene of E. coli, however, could not complement the cold-sensitive phenotype of the CS1 mutant. Interestingly, the CS1 strain showed greater sensitivity toward the DNA-damaging agents, mitomycin C and UV. The inactivation of recD in P. syringae also led to cell death and accumulation of DNA fragments of approximately 25-30 kbp in size at low temperature (4 degrees ). We propose that during growth at a very low temperature the Antarctic P. syringae is subjected to DNA damage, which requires direct participation of a unique RecD function. Additional results suggest that a truncated recD encoding the N-terminal segment of (1-576) amino acids is sufficient to support growth of P. syringae at low temperature.
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