ArticleJournal of virology2005
Identification of paramyxovirus V protein residues essential for STAT protein degradation and promotion of virus replication.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 75 citations in OpenAlex.
- Review
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- TRIM28 is a target for paramyxovirus V proteins.PLoS pathogens · 2025Article
- Autophagy and Respiratory Viruses: Mechanisms, Viral Exploitation, and Therapeutic Insights.Cells · 2025Review
- Accessory viral protein, V, of Newcastle Disease Virus binds dsRNA to facilitate immune evasion.Virusdisease · 2025Article
- Development of Primer Panels for Amplicon Sequencing of Human Parainfluenza Viruses Type 1 and 2.International journal of molecular sciences · 2024Article
- Molecular biology of canine parainfluenza virus V protein and its potential applications in tumor immunotherapy.Frontiers in microbiology · 2023Review
- Hazara Orthonairovirus Nucleoprotein Antagonizes Type I Interferon Production by Inhibition of RIG-I Ubiquitination.Viruses · 2022Article
- An E3 Ubiquitin Ligase Scaffolding Protein Is Proviral during Chikungunya Virus Infection in Aedes aegypti.Microbiology spectrum · 2022Article
- A Point Mutation in the Human Parainfluenza Virus Type 2 Nucleoprotein Leads to Two Separate Effects on Virus Replication.Journal of virology · 2022Article
- Viral Respiratory Pathogens and Lung Injury.Clinical microbiology reviews · 2021Review
- Human Parainfluenza Virus Type 2 V Protein Modulates Iron Homeostasis.Journal of virology · 2021Article
- Inhibition of Cavin3 Degradation by the Human Parainfluenza Virus Type 2 V Protein Is Important for Efficient Viral Growth.Frontiers in microbiology · 2020Article
- Respiratory syncytial virus nonstructural proteins 1 and 2: Exceptional disrupters of innate immune responses.PLoS pathogens · 2019Review
- Immune Modulation and Immune-Mediated Pathogenesis of Emerging Tickborne Banyangviruses.Vaccines · 2019Review
- Rab27a facilitates human parainfluenza virus type 2 growth by promoting cell surface transport of envelope proteins.Medical microbiology and immunology · 2018Article
- Human parainfluenza virus type 2 polymerase complex recognizes leader promoters of other species belonging to the genus Rubulavirus.Medical microbiology and immunology · 2017Article
- Tetherin antagonism by V proteins is a common trait among the genus Rubulavirus.Medical microbiology and immunology · 2017Article
- Human parainfluenza virus type 2 V protein inhibits induction of tetherin.Medical microbiology and immunology · 2017Article
- A Point Mutation in the RNA-Binding Domain of Human Parainfluenza Virus Type 2 Nucleoprotein Elicits Abnormally Enhanced Polymerase Activity.Journal of virology · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some paramyxovirus V proteins induce STAT protein degradation, and the amino acids essential for this process in the human parainfluenza virus type 2 (hPIV2) V protein have been studied. Various recombinant hPIV2s and cell lines constitutively expressing various mutant V proteins were generated. We found that V proteins with replacement of Cys residues of the Cys cluster were still able to bind STATs but were unable to induce their degradation. The hPIV2 V protein binds STATs via a W-(X)3-W-(X)9-W Trp motif located just upstream of the Cys cluster. Replacements of two or more Trp residues in this motif resulted in a failure to form a V/STAT2 complex. We have also identified two Phe residues of the hPIV2 V protein that are essential for STAT degradation, namely, Phe207, lying within the Cys cluster, and Phe143, in the P/V common region of the protein. Interestingly, infection of BHK cells with hPIV2 led to the specific degradation of STAT1 and not STAT2. Other evidence for the cell species specificity of hPIV2-induced STAT degradation is presented. Finally, a V-minus hPIV2, which can express only the P protein from its P gene, was generated and partially characterized. In contrast to V-minus viruses of other paramyxovirus genera, this V-minus rubulavirus was highly debilitated, and its growth even in Vero cells was very limited. The structural rubulavirus V proteins, as expected, are thus clearly important in promoting virus growth, independent of their anti-interferon (IFN) activity. Interestingly, many of the residues that are essential for anti-IFN activity, e.g., the Cys of this cluster and Phe207 within this cluster, as well as the Trp of this motif, are also essential for promoting virus growth.
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