ArticleJournal of virology2005
In-depth, longitudinal analysis of viral quasispecies from an individual triply infected with late-stage human immunodeficiency virus type 1, using a multiple PCR primer approach.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Trial
- Multiple Infection and Human Immunodeficiency Virus Superinfection Among Persons who Inject Drugs in Indonesia and Ukraine.The Journal of infectious diseases · 2022Article
- Quasispecies Analysis of SARS-CoV-2 of 15 Different Lineages during the First Year of the Pandemic Prompts Scratching under the Surface of Consensus Genome Sequences.International journal of molecular sciences · 2022Article
- Triple HIV-1 Infection Is Associated With Faster CD4Frontiers in microbiology · 2020Article
- Uncovering Virus-Virus Interactions by Unifying Approaches and Harnessing High-Throughput Tools.mSystems · 2019Article
- Multiplexed highly-accurate DNA sequencing of closely-related HIV-1 variants using continuous long reads from single molecule, real-time sequencing.Nucleic acids research · 2015Article
- Article
- Frequency of subtype B and F1 dual infection in HIV-1 positive, Brazilian men who have sex with men.Virology journal · 2012Article
- Evidences for viral strain selection in late stages of HIV infection: an analysis of Vpu alleles.The protein journal · 2012Article
- HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies.PloS one · 2012Article
- Superinfection by discordant subtypes of HIV-1 does not enhance the neutralizing antibody response against autologous virus.PloS one · 2012Article
- Multiple HIV-1 infections with evidence of recombination in heterosexual partnerships in a low risk Rural Clinical Cohort in Uganda.Virology · 2011Article
- Infection by discordant strains of HIV-1 markedly enhances the neutralizing antibody response against heterologous virus.Journal of virology · 2010Article
- Longitudinal quasispecies analysis of viral variants in HIV type 1 dually infected individuals highlights the importance of sequence identity in viral recombination.AIDS research and human retroviruses · 2010Article
- SHIV infection protects against heterologous pathogenic SHIV challenge in macaques: a gold-standard for HIV-1 vaccine development?Current HIV research · 2009Article
- HIV-1 superinfection in the antiretroviral therapy era: are seroconcordant sexual partners at risk?PloS one · 2009Article
- Molecular and contextual markers of hepatitis C virus and drug abuse.Molecular diagnosis & therapy · 2009Review
- A heteroduplex assay for the rapid detection of dual Human Immunodeficiency Virus Type 1 infections.Journal of virological methods · 2008Article
- Identifying HIV-1 dual infections.Retrovirology · 2007Review
- HIV-1 sequence evolution in vivo after superinfection with three viral strains.Retrovirology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Co-infections with more than one human immunodeficiency virus type 1 (HIV-1) subtype appear to be the source of new recombinant strains and may be commonplace in high-risk cohorts exposed to multiple subtypes. Many potential dual infections have been identified during the HIV Superinfection Study in Mbeya, Tanzania, where 600 female bar workers who are highly exposed to subtypes A, C, and D have been evaluated every 3 months for over 3 years by use of the MHAacd HIV-1 genotyping assay. Here we describe an in-depth, longitudinal analysis of the viral quasispecies in a woman who was triply infected with HIV-1 and who developed AIDS and passed away 15 months after enrollment. The MHA results obtained at 0, 3, 6, 9, and 12 months revealed dual-probe reactivities and shifts in subtype over time, indicating a potential dual infection and prompting further investigation. The multiple infection was confirmed by PCR amplification of three genome regions by a multiple primer approach, followed by molecular cloning and sequencing. A highly complex viral quasispecies was found, including several recombinant forms, with vpu/gp120 being the most diverse region. A significant fluctuation in molecular forms over time was observed, showing that the serial sample format is highly desirable, if not essential, for the identification of multiple infections. In a separate experiment, we confirmed that the detection of co-infections is more efficient with the use of multiple amplification primers to overcome the primer bias that results from the enormous diversity in the HIV-1 genome.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.