ArticleJournal of virology2005
Development of a novel system to study hepatitis delta virus genome replication.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 58 citations in OpenAlex.
- Analysis of Hepatitis D Virus in Minor Salivary Gland of Sjögren's Disease.Journal of dental research · 2023Article
- A changing landscape: Tracking and analysis of the international HDV epidemiology 1999-2020.PLOS global public health · 2023Article
- HDV Pathogenesis: Unravelling Ariadne's Thread.Viruses · 2021Review
- AAV-HDV: An Attractive Platform for the In Vivo Study of HDV Biology and the Mechanism of Disease Pathogenesis.Viruses · 2021Article
- Hepatitis Delta Virus (HDV) and Delta-Like Agents: Insights Into Their Origin.Frontiers in microbiology · 2021Review
- Innate immune recognition and modulation in hepatitis D virus infection.World journal of gastroenterology · 2020Review
- Art of Making Artificial Liver: Depicting Human Liver Biology and Diseases in Mice.Seminars in liver disease · 2020Review
- Enveloped viruses distinct from HBV induce dissemination of hepatitis D virus in vivo.Nature communications · 2019Article
- Article
- Hepatitis Delta Antigen Regulates mRNA and Antigenome RNA Levels during Hepatitis Delta Virus Replication.Journal of virology · 2019Article
- Insight into the Contribution and Disruption of Host Processes during HDV Replication.Viruses · 2018Review
- The Hepatitis Delta Virus accumulation requires paraspeckle components and affects NEAT1 level and PSP1 localization.Scientific reports · 2018Article
- In vivo models of hepatitis B and C virus infection.FEBS letters · 2016Review
- Hepatitis Delta Virus Detected in Salivary Glands of Sjögren's Syndrome Patients and Recapitulates a Sjögren's Syndrome-Like PhenotypePathogens & immunity · 2016Article
- Hepatitis delta virus: A fascinating and neglected pathogen.World journal of virology · 2015Review
- Hepatitis D Virus Replication.Cold Spring Harbor perspectives in medicine · 2015Review
- Host RNA circles and the origin of hepatitis delta virus.World journal of gastroenterology · 2014Review
- Searching for nuclear export elements in hepatitis D virus RNA.World journal of virology · 2013Article
- Arginine-rich motifs are not required for hepatitis delta virus RNA binding activity of the hepatitis delta antigen.Journal of virology · 2013Article
- Identification of a binding site for ASF/SF2 on an RNA fragment derived from the hepatitis delta virus genome.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Hepatitis delta virus (HDV) genome replication requires the virus-encoded small delta protein (deltaAg). During replication, nucleotide sequence changes accumulate on the HDV RNA, leading to the translation of deltaAg species that are nonfunctional or even inhibitory. A replication system was devised where all deltaAg was conditionally provided from a separate and unchanging source. A line of human embryonic kidney cells was stably transfected with a single copy of cDNA encoding small deltaAg, with expression under tetracycline (TET) control. Next, HDV genome replication was initiated in these cells by transfection with a mutated RNA unable to express deltaAg. Thus, replication of this RNA was under control of the TET-inducible deltaAg. In the absence of TET, there was sufficient deltaAg to allow a low level of HDV replication that could be maintained for at least 1 year. When TET was added, both deltaAg and genomic RNA increased dramatically within 2 days. With clones of such cells, designated 293-HDV, the burst of HDV RNA replication interfered with cell cycling. Within 2 days, there was a fivefold enhancement of G1/G0 cells relative to both S and G2/M cells, and by 6 days, there was extensive cell detachment and death. These findings and those of other studies that are under way demonstrate the potential applications of this experimental system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.