ArticleJournal of virology2005
Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 113 citations in OpenAlex.
- Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for coronavirus.Poultry science · 2026Article
- Discontinuous template switching generates coronavirus subgenomic RNAs from the 3' viral genome end by 5' to 3' transcription.Journal of virology · 2025Article
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- Molecular characterization of a novel variant of infectious bronchitis virus from field outbreaks in backyard chicken population of North East India.Virus genes · 2024Article
- Avian infectious bronchitis virus (AIBV) review by continent.Frontiers in cellular and infection microbiology · 2024Review
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- Global Emergence of Infectious Bronchitis Virus Variants: Evolution, Immunity, and Vaccination Challenges.Transboundary and emerging diseases · 2023Review
- Host-Genome Similarity Characterizes the Adaption of SARS-CoV-2 to Humans.Biomolecules · 2022Article
- The order of events on Avian coronavirus life cycle shapes the order of quasispecies evolution during host switches.Genetics and molecular biology · 2022Article
- Genome Sequence Variations of Infectious Bronchitis Virus Serotypes From Commercial Chickens in Mexico.Frontiers in veterinary science · 2022Article
- Current Knowledge on Infectious Bronchitis Virus Non-structural Proteins: The Bearer for Achieving Immune Evasion Function.Frontiers in veterinary science · 2022Review
- Reverse Genetics and Its Usage in the Development of Vaccine Against Poultry Diseases.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Interspecies Jumping of Bat Coronaviruses.Viruses · 2021Review
- Molecular Characterization of 4/91 Infectious Bronchitis Virus Leading to Studies of Pathogenesis and Host Responses in Laying Hens.Pathogens (Basel, Switzerland) · 2021Article
- Clinical and molecular aspects of veterinary coronaviruses.Virus research · 2021Review
- Replicase 1a gene plays a critical role in pathogenesis of avian coronavirus infectious bronchitis virus.Virology · 2020Article
- The molecular virology of coronaviruses.The Journal of biological chemistry · 2020Review
- Genetic sequence changes related to the attenuation of avian infectious bronchitis virus strain TW2575/98.Virus genes · 2020Article
- Genetic, antigenic and pathogenic characterization of avian coronaviruses isolated from pheasants (Phasianus colchicus) in China.Veterinary microbiology · 2020Article
- Successful establishment of a reverse genetic system for QX-type infectious bronchitis virus and technical improvement of the rescue procedure.Virus research · 2019Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The avian coronavirus Infectious bronchitis virus (IBV), like other coronaviruses, expresses several small nonstructural (ns) proteins in addition to those from gene 1 (replicase) and the structural proteins. These coronavirus ns genes differ both in number and in amino acid similarity between the coronavirus groups but show some concordance within a group or subgroup. The functions and requirements of the small ns gene products remain to be elucidated. With the advent of reverse genetics for coronaviruses, the first steps in elucidating their role can be investigated. We have used our reverse genetics system for IBV (R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton, J. Virol. 75:12359-12369, 2001) to investigate the requirement of IBV gene 5 for replication in vivo, in ovo, and ex vivo. We produced a series of recombinant viruses, with an isogenic background, in which complete expression of gene 5 products was prevented by the inactivation of gene 5 following scrambling of the transcription-associated sequence, thereby preventing the expression of IBV subgenomic mRNA 5, or scrambling either separately or together of the translation initiation codons for the two gene 5 products. As all of the recombinant viruses replicated very similarly to the wild-type virus, Beau-R, we conclude that the IBV gene 5 products are not essential for IBV replication per se and that they are accessory proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.