Evidence map›Paper›PMID 15956428›Full record

ArticleJournal of clinical microbiology2005

Use of the genomic subtractive hybridization technique to develop a real-time PCR assay for quantitative detection of Prevotella spp. in oral biofilm samples.

Shiori Nagashima, Akihiro Yoshida, Nao Suzuki, Toshihiro Ansai, Tadamichi Takehara

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shiori NagashimaDivision of Community Oral Health Science, Kyushu Dental College, 2-6-1 Manazuru, Kitakyushu 803-8580, Japan.
Akihiro Yoshida
Nao Suzuki
Toshihiro Ansai
Tadamichi Takehara

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genomic subtractive hybridization was used to design Prevotella nigrescens-specific primers and TaqMan probes. Based on this technique, a TaqMan real-time PCR assay was developed for quantifying four oral black-pigmented Prevotella species. The combination of real-time PCR and genomic subtractive hybridization is useful for preparing species-specific primer-probe sets for closely related species.

Indexed as

Bacteroidaceae InfectionsBiofilmsDental PlaqueDNA, BacterialDNA ProbesHumansMouthNucleic Acid HybridizationPolymerase Chain ReactionPrevotellaSpecies SpecificityTaq PolymeraseDNA, BacterialDNA ProbesTaq Polymerase

Identifiers

PMID15956428
PMCPMC1151961

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.