ArticleJournal of clinical microbiology2005
Matrix-assisted laser desorption ionization-time of flight (mass spectrometry) for hepatitis C virus genotyping.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Trial
- Consolidating the potency of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) in viral diagnosis: Extrapolating its applicability for COVID diagnosis?Trends in analytical chemistry : TRAC · 2022Review
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) proteomic profiling of cerebrospinal fluid in the diagnosis of enteroviral meningitis: a proof-of-principle study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Genotyping & diagnostic methods for hepatitis C virus: A need of low-resource countries.The Indian journal of medical research · 2018Review
- Comparison of Three Different Hepatitis C Virus Genotyping Methods: 5'NCR PCR-RFLP, Core Type-Specific PCR, and NS5b Sequencing in a Tertiary Care Hospital in South India.Journal of clinical laboratory analysis · 2017Article
- Hepatitis C in the Russian Federation: challenges and future directions.Hepatic medicine : evidence and research · 2016Review
- MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.Frontiers in microbiology · 2015Review
- Application of mass spectrometry to molecular diagnostics of viral infections.Expert review of molecular diagnostics · 2013Review
- Application of maldi-tof mass spectrometry in clinical virology: a review.The open virology journal · 2013Article
- [Mass spectrometry in the clinical microbiology laboratory].Enfermedades infecciosas y microbiologia clinica · 2012Review
- Identification of pathogens by mass spectrometry.Clinical chemistry · 2010Review
- Relation between genetic markers of drug resistance and susceptibility profile of clinical Neisseria gonorrhoeae strains.Antimicrobial agents and chemotherapy · 2008Article
- Multiplex detection of human herpesviruses from archival specimens by using matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2008Article
- Detection of ApoE E2, E3 and E4 alleles using MALDI-TOF mass spectrometry and the homogeneous mass-extend technology.Nucleic acids research · 2005Article
- Advances in mass spectrometry for the identification of pathogens.Mass spectrometry reviewsReview
- Subtyping of hepatitis C virus with high resolution mass spectrometry.Clinical mass spectrometry (Del Mar, Calif.)Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determination of the hepatitis C virus (HCV) genotype has become accepted as the standard procedure in laboratory practice. Genotype assignment helps in disease prognosis and assists in establishing the appropriate duration of treatment. More than 10 types and 70 subtypes of HCV have been described. In Russia the most common subtypes are 1a, 1b, 2a, and 3a, and the types 4 and 5 are relatively rare. The "gold standard" for testing is gene sequencing. However, a variety of other assays had been developed to provide more rapid and cheaper forms of testing. The aim of this study was to determine the HCV genotype by minisequencing followed by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Fragments of 5' untranslated region of the HCV genome were amplified. Three oligonucleotide primers were designed to detect two sets of genotype-specific single nucleotide polymorphisms. The primer extension reaction was performed using modified thermostable DNA polymerase and in the presence of dideoxynucleosides. The molecular weights of the reaction products were analyzed with MALDI-TOF mass spectrometer. The HCV genotype was determined by registering the particles of the expected molecular weights. The method was used to genotype HCV from HCV-positive blood sera or plasma. The 1a, 1b, 2a, 3a, and 4 genotype HCVs were determined in the samples examined. The data were confirmed by direct sequencing. Thus, we propose a new accurate and efficient method for HCV genotyping based on minisequencing followed by mass spectrometry.
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