Evidence map›Paper›PMID 15956391›Full record

ArticleJournal of clinical microbiology2005

Characterization of Salmonella enterica serovar Typhimurium and monophasic Salmonella serovar 1,4,[5],12:i:- isolates in Thailand.

P Amavisit, W Boonyawiwat, A Bangtrakulnont

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
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  3. A Multidrug-resistant MonophasicInfection and drug resistance · 2020
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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

3 authors.

P AmavisitDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand 10900. fvetpaa@ku.ac.th
W Boonyawiwat
A Bangtrakulnont

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Duplex PCR was developed to screen Salmonella enterica serovar Typhimurium phage type DT104 and related strains in Thailand because a phage typing laboratory of serovar Typhimurium is not available. Of 46 isolates of serovar Typhimurium and 32 isolates of S. enterica serovar 1,4,[5],12:i:-, 15 (33%) and 30 (94%) were duplex PCR positive, respectively. All isolates were submitted for phage typing to analyze the specificity of the PCR assay. Among serovar Typhimurium isolates that yielded positive duplex PCRs, only seven isolates were phage types DT104 or U302, and eight isolates were undefined types, whereas the negative PCR isolates were either other phage types, including DT7, DT12, DT66, DT79, DT166, DT170, DT193, and DT208 or an undefined type. The serovar Typhimurium and serovar 1,4,[5],12:i:- isolates that were duplex PCR positive were further subtyped by using XbaI PFGE to reveal their genetic relatedness. All serovar Typhimurium phage type DT104 strains had indistinguishable chromosomal patterns. The isolates of phage type U302 and most of the serovar 1,4,[5],12:i:- isolates that were duplex PCR positive yielded similar pulsed-field gel electrophoresis patterns. The patterns of PCR-negative isolates distinctly differed from the patterns of PCR-positive isolates. A total of 26% of all isolates had a dominant R-type ACSSuTG that was not found in the isolates of phage type DT104.

Indexed as

AnimalsBacterial Typing TechniquesBacteriophage TypingDNA, BacterialDrug Resistance, Multiple, BacterialFood MicrobiologyHumansPolymerase Chain ReactionSalmonella entericaSalmonella InfectionsSalmonella Infections, AnimalSalmonella typhimuriumSerotypingThailandDNA, Bacterial

Identifiers

PMID15956391
PMCPMC1151962

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.