ArticleJournal of clinical microbiology2005
Novel PCR-restriction fragment length polymorphism method for determining serotypes or serogroups of Streptococcus pneumoniae isolates.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- The Molecular Approaches and Challenges ofPolish journal of microbiology · 2023Review
- Identification of Pneumococcal Serotypes by PCR-Restriction Fragment Length Polymorphism.Diagnostics (Basel, Switzerland) · 2019Article
- Spontaneous pneumococcal peritonitis diagnosed by qPCR.IDCases · 2019Article
- Development of PCRSeqTyping-a novel molecular assay for typing ofPneumonia (Nathan Qld.) · 2017Article
- Article
- Pneumococcal Capsules and Their Types: Past, Present, and Future.Clinical microbiology reviews · 2015Review
- Comparison of a Real-Time Multiplex PCR and Sequetyping Assay for Pneumococcal Serotyping.PloS one · 2015Article
- Revisiting molecular serotyping of Streptococcus pneumoniae.BMC genomics · 2015Article
- Discovery of Streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating units.The Journal of biological chemistry · 2013Article
- Serotyping of Streptococcus pneumoniae isolated from Tehran by Multiplex PCR: Are serotypes of clinical and carrier isolates identical?Iranian journal of microbiology · 2013Article
- Article
- From Quellung to multiplex PCR, and back when needed, in pneumococcal serotyping.Journal of clinical microbiology · 2012Article
- Sequetyping: serotyping Streptococcus pneumoniae by a single PCR sequencing strategy.Journal of clinical microbiology · 2012Article
- Development of an automated and multiplexed serotyping assay for Streptococcus pneumoniae.Clinical and vaccine immunology : CVI · 2011Article
- A new microarray system to detect Streptococcus pneumoniae serotypes.Journal of biomedicine & biotechnology · 2011Article
- Simple, accurate, serotype-specific PCR assay to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C.Journal of clinical microbiology · 2009Article
- Article
- Use of the Agilent 2100 bioanalyzer for rapid and reproducible molecular typing of Streptococcus pneumoniae.Journal of clinical microbiology · 2007Article
- Validation of a multiplex pneumococcal serotyping assay with clinical samples.Journal of clinical microbiology · 2006Article
- Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serotyping Streptococcus pneumoniae is a technique generally confined to reference laboratories, as purchasing pneumococcal antisera is a huge investment. Many attempts have been made to modify serological agglutination techniques to make them more accessible, and more recently developments in serotyping have focused on molecular techniques. This paper describes a PCR assay which amplifies the entire capsulation locus between dexB and aliA. Amplicons are digested to produce serotype-specific patterns. We have shown, using 81 epidemiologically unrelated strains representing 46 different serotypes, that the patterns correlate with a 90 to 100% similarity range for the same serotype or serogroup. Prospective testing of 73 isolates of unknown serotype confirmed reliable serotype attribution, and serotype profiles are reproducible on repeated testing. Once our database contains all 90 serotypes, this technique should be fully portable, cost-effective, and useful in any laboratory with sufficient molecular experience.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.