ArticleJournal of clinical microbiology2005
Use of the INNO-LiPA-MYCOBACTERIA assay (version 2) for identification of Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex isolates.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Guideline
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- PCR-reverse blot hybridization assay in respiratory specimens for rapid detection and differentiation of mycobacteria in HIV-negative population.BMC infectious diseases · 2021Article
- Target-Specific Assay for Rapid and Quantitative Detection of Mycobacterium chimaera DNA.Journal of clinical microbiology · 2017Article
- Standardization of a TaqMan-based real-time PCR for the detection of Mycobacterium tuberculosis-complex in human sputum.The American journal of tropical medicine and hygiene · 2014Article
- Evaluation of the speed-oligo direct Mycobacterium tuberculosis assay for molecular detection of mycobacteria in clinical respiratory specimens.Journal of clinical microbiology · 2013Article
- An Update on Global Tuberculosis (TB).Infectious diseases · 2013Review
- Multi-centre evaluation of the speed-oligo Mycobacteria assay for differentiation of Mycobacterium spp. in clinical isolates.BMC infectious diseases · 2011Article
- Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Evaluation of Cobas TaqMan MTB PCR for detection of Mycobacterium tuberculosis.Journal of clinical microbiology · 2011Article
- Rapid Identification of Mycobacterium tuberculosis and nontuberculous mycobacteria by multiplex, real-time PCR.Journal of clinical microbiology · 2009Article
- Characterization of "Mycobacterium paraffinicum" associated with a pseudo-outbreak.Journal of clinical microbiology · 2008Article
- Lymphadenopathy caused by Mycobacterium colombiense.Journal of clinical microbiology · 2008Article
- Mycobacterium avium in the postgenomic era.Clinical microbiology reviews · 2007Review
- Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 mycobacterium species.Journal of clinical microbiology · 2006Article
- Sequencing of hsp65 distinguishes among subsets of the Mycobacterium avium complex.Journal of clinical microbiology · 2006Article
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Authors and funding
10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Using INNO-LiPA-MYCOBACTERIA (Lipav1; Innogenetics) and the AccuProbe (Gen-Probe Inc./bioMérieux) techniques, 35 Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum (MAC/MAIS) complex strains were identified between January 2000 and December 2002. Thirty-four of 35 isolates were positive only for the MAIS complex probe by Lipav1 and were further analyzed by INNO-LiPA-MYCOBACTERIA version 2 (Lipav2), hsp65 PCR restriction pattern analysis (PRA), and ribosomal internal transcribed spacer (ITS), hsp65, and 16S rRNA sequences. Lipav2 identified 14 of 34 strains at the species level, including 11 isolates positive for the newly specific MAC sequevar Mac-A probe (MIN-2 probe). Ten of these 11 isolates corresponded to sequevar Mac-A, which was recently defined as Mycobacterium chimerae sp. nov. Among the last 20 of the 34 MAIS isolates, 17 (by hsp65 PRA) and 18 (by hsp65 sequence) were characterized as M. avium. Ten of the 20 were identified as Mac-U sequevar. All these 20 isolates were identified as M. intracellulare by 16S rRNA sequence except one isolate identified as Mycobacterium paraffinicum by 16S rRNA and ITS sequencing. One isolate out of 35 isolates that was positive for M. avium by AccuProbe and that was Mycobacterium genus probe positive and MAIS probe negative by Lipav1 and Lipav2 might be considered a new species. In conclusion, the new INNO-LiPA-MYCOBACTERIA allowed the identification of 40% of the previously unidentified MAIS isolates at the species level. The results of the Lipav2 assay on the MAIS isolates confirm the great heterogeneity of this group and suggest the use of hsp65 or ITS sequencing for precise identification of such isolates.
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