ArticlePlant physiology2005
Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed.
- Spatiotemporal regulation of arbuscular mycorrhizal symbiosis at cellular resolution.The Plant cell · 2026Article
- Setting a TRAP for phloem translational dynamics during nodulation.Plant physiology · 2025Article
- Phloem-specific translational regulation of soybean nodulation: Insights from a phloem-targeted TRAP-seq approach.Plant physiology · 2025Article
- Arabidopsis as a model for translational research.The Plant cell · 2025Review
- A timeline of discovery and innovation in Arabidopsis.The Plant cell · 2025Article
- RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sites.Nature communications · 2025Article
- The transcriptional integration of environmental cues with root cell type development.Plant physiology · 2024Review
- Florigen-producing cells express FPF1-LIKE PROTEIN 1 that accelerates flowering and stem growth in long days with sunlight red/far-red ratio inbioRxiv : the preprint server for biology · 2024Article
- Plant mRNAs move into a fungal pathogen via extracellular vesicles to reduce infection.Cell host & microbe · 2024Article
- Legume-rhizobia symbiosis: Translatome analysis.Genetics and molecular biology · 2024Article
- Isolation of Cytosolic Ribosomes Associated with Plant Mitochondria and Chloroplasts.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis.Nature plants · 2023Article
- Arabidopsis telomerase takes off by uncoupling enzyme activity from telomere length maintenance in space.Nature communications · 2023Article
- Review
- Fast neurotransmitter identity of MCH neurons: Do contents depend on context?Frontiers in neuroendocrinology · 2023Review
- The translational landscape of bread wheat during grain development.The Plant cell · 2023Article
- Specific alterations in riboproteomes composition of isonicotinic acid treated arabidopsis seedlings.Plant molecular biology · 2023Article
- Laser Microdissection of Woody and Suberized Plant Tissues for RNA-Seq Analysis.Molecular biotechnology · 2023Article
- Regulatory miPEP Open Reading Frames Contained in the Primary Transcripts of microRNAs.International journal of molecular sciences · 2023Review
- FRIENDLY (FMT) is an RNA binding protein associated with cytosolic ribosomes at the mitochondrial surface.The Plant journal : for cell and molecular biology · 2022Article
45 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts of Arabidopsis (Arabidopsis thaliana) was achieved with transgenic genotypes that overexpress a translational fusion of a ribosomal protein (RP) with a His(6)-FLAG dual epitope tag. In plants with a cauliflower mosaic virus 35S:HF-RPL18 transgene immunopurification with anti-FLAG agarose beads yielded 60-Svedberg ribosomal subunits, intact 80-Svedberg monosomes and polysomes. Sucrose density gradient fractionation of the purified complexes demonstrated that the distribution of polysome size was similar in crude cell extracts and the purified complexes. The immunopurified complexes included putative cytosolic RPs of Arabidopsis and ribosome-associated proteins, as well as full-length transcripts of high and low abundance. Whole-genome profiling using long DNA oligonucleotide-based microarrays provided a high level of reproducibility between polysomal mRNA samples immunopurified from two independent biological replicates (r approximately 0.90). Comparison of immunopurified and total cellular RNA samples revealed that for most of the genes, the mRNAs were associated with the epitope-tagged polysomal complexes, with an average relative level of association of 62.06% +/- 4.39%. The results demonstrate that the immunopurification of polysomes can be a valuable tool for the quantification of mRNAs present in translation complexes in plant cells. This technology can be extended to evaluation of mRNA populations at the cell- or tissue-specific level by regulation of the tagged RP with distinct promoters.
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