ArticleExperimental parasitology2005
Species- and strain-specific probes derived from repetitive DNA for distinguishing Entamoeba histolytica and Entamoeba dispar.
Article in Experimental parasitology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Molecular Epidemiology of Amoebiasis: A Cross-Sectional Study among North East Indian Population.PLoS neglected tropical diseases · 2015Article
- Systematic detection and association of Entamoeba species in stool samples from selected sites in India.Epidemiology and infection · 2015Article
- The trend in distribution of Q223R mutation of leptin receptor gene in amoebic liver abscess patients from North India: a prospective study.BioMed research international · 2014Article
- Differential expression and immunolocalization of antioxidant enzymes in Entamoeba histolytica isolates during metronidazole stress.BioMed research international · 2014Article
- Genomic distribution of SINEs in Entamoeba histolytica strains: implication for genotyping.BMC genomics · 2013Article
- Real-time analysis of gut flora in Entamoeba histolytica infected patients of Northern India.BMC microbiology · 2012Article
- Bioinformatic analysis of Entamoeba histolytica SINE1 elements.BMC genomics · 2010Article
- Molecular methods for diagnosis of Entamoeba histolytica in a clinical setting: an overview.Experimental parasitology · 2007Review
- Involvement of a short interspersed element in epigenetic transcriptional silencing of the amoebapore gene in Entamoeba histolytica.Eukaryotic cell · 2005Article
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3 authors.
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Abstract
Entamoeba histolytica and Entamoeba dispar are two morphologically indistinguishable species that are found in the human gut. Of the two, E. histolytica is considered to be pathogenic while E. dispar is nonpathogenic. To generate molecular probes to detect and distinguish between the two species, we utilized repeat sequences present in Entamoeba genome. We have developed probes and primers from rDNA episomes, and unidentified Entamoeba EST1 repeat for this purpose, and used them for dot blot hybridization and PCR amplification. To investigate the possible existence of invasive and noninvasive strains of E. histolytica, the ability to differentiate individual isolates is necessary. For this purpose, we have utilized a modification of the AFLP procedure called 'Transposon display,' which generates and displays large number of genomic bands associated with a transposon. We have used the abundant retrotransposon, EhSINE1, for this purpose,and demonstrated its potential as a marker to study strain variation in E. histolytica. This technique could suitably be employed in carrying out significant molecular epidemiological studies and large-scale typing of this parasite.
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