ArticlePlant molecular biology2005
CaaX-prenyltransferases are essential for expression of genes involvedin the early stages of monoterpenoid biosynthetic pathway in Catharanthus roseus cells.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 45 citations in OpenAlex.
- Methyl-Jasmonate Functions as a Molecular Switch Promoting Cross-Talk between Pathways for the Biosynthesis of Isoprenoid Backbones Used to Modify Proteins in Plants.Plants (Basel, Switzerland) · 2024Article
- Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis.The Biochemical journal · 2023Article
- Cellular and Subcellular Compartmentation of the 2Plants (Basel, Switzerland) · 2020Article
- Dynamics of Monoterpene Formation in Spike Lavender Plants.Metabolites · 2017Article
- Folivory elicits a strong defense reaction in Catharanthus roseus: metabolomic and transcriptomic analyses reveal distinct local and systemic responses.Scientific reports · 2017Article
- Biosynthetic pathway of terpenoid indole alkaloids in Catharanthus roseus.Frontiers of medicine · 2014Review
- S-carvone suppresses cellulase-induced capsidiol production in Nicotiana tabacum by interfering with protein isoprenylation.Plant physiology · 2014Article
- Transcriptome analysis reveals putative genes involved in iridoid biosynthesis in Rehmannia glutinosa.International journal of molecular sciences · 2012Article
- A single gene encodes isopentenyl diphosphate isomerase isoforms targeted to plastids, mitochondria and peroxisomes in Catharanthus roseus.Plant molecular biology · 2012Article
- Molecular cloning and functional characterization of Catharanthus roseus hydroxymethylbutenyl 4-diphosphate synthase gene promoter from the methyl erythritol phosphate pathway.Molecular biology reports · 2012Article
- A virus-induced gene silencing approach to understanding alkaloid metabolism in Catharanthus roseus.Phytochemistry · 2011Article
- Strictosidine activation in Apocynaceae: towards a "nuclear time bomb"?BMC plant biology · 2010Article
- Silencing of tryptamine biosynthesis for production of nonnatural alkaloids in plant culture.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Optimization of the transient transformation of Catharanthus roseus cells by particle bombardment and its application to the subcellular localization of hydroxymethylbutenyl 4-diphosphate synthase and geraniol 10-hydroxylase.Plant cell reports · 2009Article
- Proteins prenylated by type I protein geranylgeranyltransferase act positively on the jasmonate signalling pathway triggering the biosynthesis of monoterpene indole alkaloids in Catharanthus roseus.Plant cell reports · 2009Article
- Spatial distribution and hormonal regulation of gene products from methyl erythritol phosphate and monoterpene-secoiridoid pathways in Catharanthus roseus.Plant molecular biology · 2007Article
- Epidermis is a pivotal site of at least four secondary metabolic pathways in Catharanthus roseus aerial organs.Planta · 2006Article
- Overexpression of farnesyl diphosphate synthase in Arabidopsis mitochondria triggers light-dependent lesion formation and alters cytokinin homeostasis.Plant molecular biology · 2006Article
- CaaX-prenyltransferases are essential for expression of genes involvedin the early stages of monoterpenoid biosynthetic pathway in Catharanthus roseus cells.Plant molecular biology · 2005Article
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8 authors at 1 institution in 1 country.
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Abstract
CaaX-prenyltransferases (CaaX-PTases) catalyse the covalent attachment of isoprenyl groups to conserved cysteine residues located at the C-terminal CaaX motif of a protein substrate. This post-translational modification is required for the function and/or subcellular localization of some transcription factors and components of signal transduction and membrane trafficking machinery. CaaX-PTases, including protein farnesyltransferase (PFT) and type-I protein geranylgeranyltransferase (PGGT-I), are heterodimeric enzymes composed of a common alpha subunit and a specific beta subunit. We have established RNA interference cell lines targeting the beta subunits of PFT and PGGT-I, respectively, in the Catharanthus roseus C20D cell line, which synthesizes monoterpenoid indole alkaloids in response to auxin depletion from the culture medium. In both types of RNAi cell lines, expression of a subset of genes involved in the early stage of monoterpenoid biosynthetic pathway (ESMB genes), including the MEP pathway, is strongly decreased. The role of CaaX-PTases in ESMB gene regulation was confirmed by using the general prenyltransferase inhibitor s-perillyl alcohol (SP) and the specific PFT inhibitor Manumycin A on the wild type line. Furthermore, supplementation of SP inhibited cells with monoterpenoid intermediates downstream of the steps encoded by the ESMB genes restores monoterpenoid indole alkaloids biosynthesis. We conclude that protein targets for both PFT and PGGT-I are required for the expression of ESMB genes and monoterpenoid biosynthesis in C. roseus, this represents a non previously described role for protein prenyltransferase in plants.
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