ArticleGut2005
Growth, phenotype, and function of human intestinal mast cells are tightly regulated by transforming growth factor beta1.
Article in Gut, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 78 citations in OpenAlex.
- Article
- Inhibitory Effects ofPlants (Basel, Switzerland) · 2022Article
- Utilizing mast cells in a positive manner to overcome inflammatory and allergic diseases.Frontiers in immunology · 2022Review
- Electron microscopy of juvenile nasopharyngeal angiofibroma: clinical and histopathological correlates.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2022Article
- Article
- Novel Strategies to Target Mast Cells in Disease.Journal of innate immunity · 2021Review
- Endoglin: An 'Accessory' Receptor Regulating Blood Cell Development and Inflammation.International journal of molecular sciences · 2020Review
- Mast Cell Regulation and Irritable Bowel Syndrome: Effects of Food Components with Potential Nutraceutical Use.Molecules (Basel, Switzerland) · 2020Review
- An In Vitro Model of Mast Cell Recruitment and Activation by Breast Cancer Cells Supports Anti-Tumoral Responses.International journal of molecular sciences · 2020Article
- Beyond IgE: Alternative Mast Cell Activation Across Different Disease States.International journal of molecular sciences · 2020Review
- Mast Cells in Liver Fibrogenesis.Cells · 2019Review
- Chronic Stress, Inflammation, and Colon Cancer: A CRH System-Driven Molecular Crosstalk.Journal of clinical medicine · 2019Review
- Article
- Mast Cell, the Neglected Member of the Tumor Microenvironment: Role in Breast Cancer.Journal of immunology research · 2018Review
- Regulation of the Immune Response by TGF-β: From Conception to Autoimmunity and Infection.Cold Spring Harbor perspectives in biology · 2017Review
- The relationship between breast cancer molecular subtypes and mast cell populations in tumor microenvironment.Virchows Archiv : an international journal of pathology · 2017Article
- Interferon-γ regulates growth and controls Fcγ receptor expression and activation in human intestinal mast cells.BMC immunology · 2014Article
- Perinatal stress, brain inflammation and risk of autism-review and proposal.BMC pediatrics · 2012Review
- Brief report: "allergic symptoms" in children with Autism Spectrum Disorders. More than meets the eye?Journal of autism and developmental disorders · 2011Review
- Phenotypic and functional plasticity of cells of innate immunity: macrophages, mast cells and neutrophils.Nature immunology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsTransforming growth factor beta1 (TGF-beta1) is expressed in the healthy human intestine and controls mucosal immune responses and inflammation by regulating the function of lymphocytes, macrophages, dendritic cells, and eosinophils. Here, we asked whether human intestinal mast cells (MC) might also be subject to immunoregulation by TGF-beta1.
methodsMC were isolated from the intestinal mucosa, purified, and cultured in vitro in the presence of stem cell factor (SCF) with or without TGF-beta1. Growth characteristics, phenotype, and immunological mediator release of MC were analysed by reverse transcription-polymerase chain reaction, flow cytometry, immunocytochemistry, western blot, and different immunoassays, respectively.
resultsTGF-beta1 dose dependently (ED50 approximately = 0.1 ng/ml) inhibited SCF dependent growth of human intestinal MC by both enhancing apoptosis and decreasing proliferation. In parallel, TGF-beta1 increased the percentage of connective tissue-type MC expressing tryptase and chymase while downregulating expression of the receptors for IgE and SCF. Furthermore, TGF-beta1 dramatically changed the profile of mediators released by MC following IgE receptor crosslinking. Release of histamine, cysteinyl-leukotrienes, and tumour necrosis factor alpha was strongly reduced whereas prostaglandin D2 generation and cyclooxygenase 1 and 2 expression were upregulated by TGF-beta1.
conclusionsOur data indicate that TGF-beta1 acts as a novel potent inhibitor and modulator of human intestinal MC effector functions. The change in MC mediator release profile and protease expression induced by TGF-beta1 might be of relevance for the control of MC associated disorders of the intestine such as allergic reactions, Crohn's disease, irritable bowel syndrome, and parasitic infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.