ArticleThe Plant cell2005
Inhibition of de novo pyrimidine synthesis in growing potato tubers leads to a compensatory stimulation of the pyrimidine salvage pathway and a subsequent increase in biosynthetic performance.
Article in The Plant cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 94 citations in OpenAlex.
- Strategic global data integration to improve genomic prediction accuracy in tree breeding programs facing resource limitations, a case study in mango.Horticulture research · 2026Article
- Uridine Kinase-like Protein (GhUKL4) Positively Regulates Resistance to Verticillium Wilt in Cotton.Genes · 2025Article
- Topological criterion for robust perfect adaptation of reaction fluxes in biological networks.iScience · 2025Article
- Induced responses to the wheat pathogen: Tan Spot-(Pyrenophora tritici-repentis) in wheat (Triticum aestivum) focus on changes in defence associated and sugar metabolism.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings.Frontiers in plant science · 2023Article
- Mechanisms of feedback inhibition and sequential firing of active sites in plant aspartate transcarbamoylase.Nature communications · 2021Article
- CTP Synthase 2 FromFrontiers in plant science · 2021Article
- SNPeffect: identifying functional roles of SNPs using metabolic networks.The Plant journal : for cell and molecular biology · 2020Article
- Synchronization of developmental, molecular and metabolic aspects of source-sink interactions.Nature plants · 2020Review
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- Endophyte-Promoted Phosphorus Solubilization inFrontiers in plant science · 2020Article
- WHITE STRIPE LEAF8, encoding a deoxyribonucleoside kinase, is involved in chloroplast development in rice.Plant cell reports · 2020Article
- Pyrimidine Salvage: Physiological Functions and Interaction with Chloroplast Biogenesis.Plant physiology · 2019Article
- UMP Kinase Regulates Chloroplast Development and Cold Response in Rice.International journal of molecular sciences · 2019Article
- Regulation of the Central Carbon Metabolism in Apple Fruit Exposed to Postharvest Low-Oxygen Stress.Frontiers in plant science · 2019Review
- Starch Trek: The Search for Yield.Frontiers in plant science · 2018Review
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- Osmosensitive changes of carbohydrate metabolism in response to cellulose biosynthesis inhibition.Plant physiology · 2012Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrimidine nucleotides are of general importance for many aspects of cell function, but their role in the regulation of biosynthetic processes is still unclear. In this study, we investigate the influence of a decreased expression of UMP synthase (UMPS), a key enzyme in the pathway of de novo pyrimidine synthesis, on biosynthetic processes in growing potato (Solanum tuberosum) tubers. Transgenic plants were generated expressing UMPS in the antisense orientation under the control of the tuber-specific patatin promoter. Lines were selected with markedly decreased expression of UMPS in the tubers. Decreased expression of UMPS restricted the use of externally supplied orotate for de novo pyrimidine synthesis in tuber tissue, whereas the uridine-salvaging pathway was stimulated. This shift in the pathways of UMP synthesis was accompanied by increased levels of tuber uridine nucleotides, increased fluxes of [(14)C]sucrose to starch and cell wall synthesis, and increased amounts of starch and cell wall components in the tubers, whereas there were no changes in uridine nucleotide levels in leaves. Decreased expression of UMPS in tubers led to an increase in transcript levels of carbamoylphosphate synthase, uridine kinase, and uracil phosphoribosyltransferase, the latter two encoding enzymes in the pyrimidine salvage pathways. Thus, the results show that antisense inhibition of the de novo pathway of pyrimidine synthesis leads to a compensatory stimulation of the less energy-consuming salvage pathways, probably via increased expression and activity of uridine kinase and uracil phosphoribosyltransferase. This results in increased uridine nucleotide pool levels in tubers and improved biosynthetic performance.
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