ArticleExperimental cell research2005
Chinese hamster ORC subunits dynamically associate with chromatin throughout the cell-cycle.
Article in Experimental cell research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- ORC6 acts as a biomarker and reflects poor outcome in clear cell renal cell carcinoma.Journal of Cancer · 2022Article
- Multiple, short protein binding motifs in ORC1 and CDC6 control the initiation of DNA replication.Molecular cell · 2021Article
- A new class of disordered elements controls DNA replication through initiator self-assembly.eLife · 2019Article
- Physiological effects of a novel artificially synthesized antimalarial cyclic peptide: Mahafacyclin B.PloS one · 2017Article
- Eukaryotic replication origins: Strength in flexibility.Nucleus (Austin, Tex.) · 2016Review
- Dynamic loading and redistribution of the Mcm2-7 helicase complex through the cell cycle.The EMBO journal · 2015Article
- The contribution of dormant origins to genome stability: from cell biology to human genetics.DNA repair · 2014Review
- Multi-step loading of human minichromosome maintenance proteins in live human cells.The Journal of biological chemistry · 2013Article
- Efficient replication of Epstein-Barr virus-derived plasmids requires tethering by EBNA1 to host chromosomes.Journal of virology · 2013Article
- Relationship between DNA replication and the nuclear matrix.Genes to cells : devoted to molecular & cellular mechanisms · 2013Review
- The dynamics of replication licensing in live Caenorhabditis elegans embryos.The Journal of cell biology · 2012Article
- Assembly of the transcription machinery: ordered and stable, random and dynamic, or both?Chromosoma · 2011Review
- Different roles of the human Orc6 protein in the replication initiation process.Cellular and molecular life sciences : CMLS · 2011Article
- Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.Nucleic acids research · 2011Article
- Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload.The Journal of cell biology · 2011Article
- The origin recognition complex protein family.Genome biology · 2009Review
- Binding of Drosophila ORC proteins to anaphase chromosomes requires cessation of mitotic cyclin-dependent kinase activity.Molecular and cellular biology · 2009Article
- Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatin.The EMBO journal · 2007Article
- A structural role for ATP in the formation and stability of the human origin recognition complex.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In yeast, the Origin Recognition Complex (ORC) is bound to replication origins throughout the cell-cycle, but in animal cells, there are conflicting data as to whether and when ORC is removed from chromatin. We find ORC1, 2 and ORC4 to be metabolically stable proteins that co-fractionate with chromatin throughout the cell-cycle in Chinese hamster fibroblasts. Since cellular extraction methods cannot directly examine the chromatin binding properties of proteins in vivo, we examined ORC:chromatin interactions in living cells. Fluorescence loss in photobleaching (FLIP) studies revealed ORC1 and ORC4 to be highly dynamic proteins during the cell-cycle with exchange kinetics similar to other regulatory chromatin proteins. In vivo interaction with chromatin was not significantly altered throughout the cell-cycle, including S-phase. These data support a model in which ORC subunits dynamically interact with chromatin throughout the cell-cycle.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.