ArticleThe Biochemical journal2005
Carbonic anhydrase (Nce103p): an essential biosynthetic enzyme for growth of Saccharomyces cerevisiae at atmospheric carbon dioxide pressure.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 90 citations in OpenAlex.
- Physiological Roles of Carbonic Anhydrases and an MpsAB-like Bicarbonate Transporter inbioRxiv : the preprint server for biology · 2026Article
- Rorippa islandica: a genetically accessible dicot model system to study flooding tolerance.Plant physiology · 2026Article
- Elevated carbon dioxide enhances the growth and reduces the antifungal susceptibility ofMicrobiology spectrum · 2025Article
- Increased CONature communications · 2024Article
- Carbonic Anhydrase Inhibition as a Target for Antibiotic Synergy in Enterococci.Microbiology spectrum · 2023Article
- LCIB functions as a carbonic anhydrase: evidence from yeast and Arabidopsis carbonic anhydrase knockout mutants.Photosynthesis research · 2023Article
- Pyrrolyl and Indolyl α-γ-Diketo Acid Derivatives Acting as Selective Inhibitors of Human Carbonic Anhydrases IX and XII.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Trajectories for the evolution of bacterial COProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Arabidopsis plastid carbonic anhydrase βCA5 is important for normal plant growth.Plant physiology · 2022Article
- A Rapid Method for Detecting Normal or Modified Plant and Algal Carbonic Anhydrase Activity UsingPlants (Basel, Switzerland) · 2022Article
- Absence of carbonic anhydrase in chloroplasts affects CProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Functional reconstitution of a bacterial COeLife · 2020Article
- Two flavonoid-based compounds fromJournal of microbiology and biotechnology · 2020Article
- Genomic and Physiological Characteristics of a Novel Nitrite-OxidizingFrontiers in microbiology · 2020Article
- DABs are inorganic carbon pumps found throughout prokaryotic phyla.Nature microbiology · 2019Article
- The molecular biology of fruity and floral aromas in beer and other alcoholic beverages.FEMS microbiology reviews · 2019Review
- Reassessment of requirements for anaerobic xylose fermentation by engineered, non-evolved Saccharomyces cerevisiae strains.FEMS yeast research · 2019Article
- Polymorphisms inFrontiers in microbiology · 2019Article
- The COVeterinary research · 2018Article
- Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors.AMB Express · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NCE103 gene of the yeast Saccharomyces cerevisiae encodes a CA (carbonic anhydrase) that catalyses the interconversion of CO2 and bicarbonate. It has previously been reported that nce103 null mutants require elevated CO2 concentrations for growth in batch cultures. To discriminate between 'sparking' effects of CO2 and a CO2 requirement for steady-state fermentative growth, we switched glucose-limited anaerobic chemostat cultures of an nce103 null mutant from sparging with pure CO2 to sparging with nitrogen gas. This switch resulted in wash-out of the biomass, demonstrating that elevated CO2 concentrations are required even under conditions where CO2 is produced at high rates by fermentative sugar metabolism. Nutritional analysis of the nce103 null mutant demonstrated that growth on glucose under a non-CO2-enriched nitrogen atmosphere was possible when the culture medium was provided with L-aspartate, fatty acids, uracil and L-argininine. Thus the main physiological role of CA during growth of S. cerevisiae on glucose-ammonium salts media is the provision of inorganic carbon for the bicarbonate-dependent carboxylation reactions catalysed by pyruvate carboxylase, acetyl-CoA carboxylase and CPSase (carbamoyl-phosphate synthetase). To our knowledge, the present study represents the first full determination of the nutritional requirements of a CA-negative organism to date.
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