ArticlePharmaceutical research2005
Vascular endothelium-selective gene induction by Tie2 promoter/enhancer in the brain and retina of a transgenic rat.
Article in Pharmaceutical research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 18 citations in OpenAlex.
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- Endothelial NMDA receptors mediate activity-dependent brain hemodynamic responses in mice.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Real-time Imaging of an Experimental Intracranial Aneurysm in Rats.Neurologia medico-chirurgica · 2019Article
- Brain endothelial cell junctions after cerebral hemorrhage: Changes, mechanisms and therapeutic targets.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018Review
- TGF-β signaling mediates endothelial-to-mesenchymal transition (EndMT) during vein graft remodeling.Science translational medicine · 2014Article
- Knockdown of interleukin-1 receptor type-1 on endothelial cells attenuated stress-induced neuroinflammation and prevented anxiety-like behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Targeting microvasculature for neuroprotection after SCI.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2011Review
- Endothelial IL-1R1 is a critical mediator of EAE pathogenesis.Brain, behavior, and immunity · 2011Article
- Astrocyte- and endothelial-targeted CCL2 conditional knockout mice: critical tools for studying the pathogenesis of neuroinflammation.Journal of molecular neuroscience : MN · 2009Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo produce a transgenic rat harboring the Tie2 promoter/enhancer linked green fluorescence protein (GFP) gene and investigate the blood-brain barrier (BBB)- and inner blood-retinal barrier (iBRB)-selective GFP expression in the brain and retina.
methodsTransgenic rats were produced by the microinjection of mouse Tie2/GFP gene into fertilized oocytes of Wistar rats. The expression of GFP was observed in tissue slices by confocal microscopy.
resultsOne of the obtained transgenic lines showed intense GFP expression in vascular endothelial cells throughout the brain. After double immunostaining with glucose transporter 1, the GFP was found to be localized in the cytoplasmic compartment of brain capillary endothelial cells. In contrast, no fluorescence was observed in neural cells. In the retina of transgenic rats, intense GFP expression was detected in retinal capillary endothelial cells. No fluorescence was detected in other cells. In kidney and liver, GFP expression was detected in vascular endothelial cells, whereas the expressed region was limited.
conclusionsMouse Tie2 promoter/enhancer has the ability to induce gene expression selectively in vascular endothelial cells in the brain and retina of transgenic rats.
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